Microarray analysis of hepatic gene expression from control,model,DSS group

ABSTRACT: Metabolic syndrome (MetS) is a pathological state of many abnormally metabolic sections. DSS is seldom reported about the application of MetS and the mechanism how DSS improves gut microbiadysbiosis and hepatic lipid homeostasis. Pharmacological actions were evaluated by comparison of transcriptomic assessments on liver tissues from fructose-fed rats. The aim of investigation was in order to find which pathways were altered by fructose and how DSS effected the metabolic pathways. Rats in control group were normally fed with food and water. Rats in model were fed with 15% fructose water and normal food. Rats in DSS group were intervened orally by Danggui-shaoyao-san extracts, besides the same fed way as model group. Total RNA was extracted from liver according to the test kit’s instruction. After a series of treatment, The cDNA was amplified and enriched by PCR and QC tested by Agilent 2100 Bioanalyzer&ABI StepOnePlus Real-Time PCR System (Agilent Technologies Inc, California). Finally the data was sequenced by Illumina platform and the reads were compared by HISAT2 software. Differential analysis was performed by DEGseq&DESeq platform. Finally, all the evidences showed that fructose could induce the differential gene changes and metabolite discordances and DSS could regulate back these abnormalities especially in carbohydrate, lipid and amino acids at the Level 2 of KEGG pathway.

ORGANISM(S): Mus musculus

PROVIDER: GSE176572 | GEO | 2021/08/03

REPOSITORIES: GEO