Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Microbiome-specific T follicular helper cells drive tertiary lympohid structures and anti-tumor immunity against colorectal cancer

ABSTRACT: Advanced or metastatic colorectal cancer is largely refractory to current therapeutic regimens and correspondingly has poor prognosis. The microbiota plays an important role in controlling the host immune response, with unique bacterial species able to activate and differenation specific subpopulations of T cells in the colon. Using an orthotopic carcinogen-induced murine model of colorectal cancer, we found that pertubation of the microbiome with Helicobacter hepaticus led to increased antitumor immunity and disease control. Single-cell RNAseq analysis was used to interrogate the tumor microenvironment and lamina propria in Helicobacter hepaticus colonized and control mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE178485 | GEO | 2021/12/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

fliA regulon of Helicobacter hepaticus

Project description:Differential gene regulation in Helicobacter hepaticus between wild type bacteria and fliA mutant

2009-07-14 | GSE12123 | GEO

Blimp-1 and c-Maf regulate common and unique gene networks to protect against distinct pathways of pathobiont-induced colitis [RNA-Seq]

Project description:Using single-cell RNA-seq of colon lamina propria leukocytes (LPL), together with ATAC-seq, ChIP-seq and RNA-seq from colon LPL and sorted CD4+ T from colon lamina propria during oral infection with the pathobiont Helicobacter hepaticus we show that Blimp-1 and c-Maf both positively regulate Il10 gene expression, but also cooperate to negatively regulate a large network of proinflammatory effector genes in T cells. While T cell-specific deletion of either Prdm1, Maf or both transcription factors did not result in overt inflammation of the colon at steady state, H. hepaticus infection resulted in mild/moderate colitis in both the Prdm1fl/flCd4Cre and Maffl/flCd4Cre single T cell-specific transcription factor deficient mice, while the double knockout Prdm1fl/flMaffl/flCd4Cre infected mice suffered severe colitis and showed a massive increase in T cell effector genes in colonic LPL. Blimp-1 and c-Maf cooperate to negatively regulate genes including Ifng, Csf2, Il23r, Stat4, Il18r1 and Il2rg, and deletion of both these transcription factors in T cells translated to an exacerbated pathogenic TH1, IFN-g+GM-CSF+ effector cell response. c-Maf, additionally negatively regulated Il17a expression which was elevated in the LPL as assessed by bulk RNA-seq and scRNA-Seq analysis in the H. hepaticus infected Maffl/flCd4Cre mice.

2024-02-18 | GSE240421 | GEO

Blimp-1 and c-Maf regulate common and unique immune gene networks to protect against distinct pathways of pathobiont-induced colitis [ATAC-Seq]

2024-02-18 | GSE240420 | GEO

Blimp-1 and c-Maf regulate common and unique immune gene networks to protect against distinct pathways of pathobiont-induced colitis [scRNA-Seq]

2024-02-18 | GSE240419 | GEO

H. hepaticus infection and liver cancer

Project description:We are investigating the transcriptional response of mice infected with Helicobacter hepaticus and links to liver cancer We used microarrays to detail the global programme of gene expression underlying H. hepaticus induced liver cancer Keywords: disease model

2007-11-01 | GSE9097 | GEO

Blimp-1 and c-Maf regulate common and unique immune gene networks to protect against distinct pathways of pathobiont-induced colitis [bulk tissue RNA-seq]

Project description:Using single-cell RNA-seq and bulk tissue RNA-seq of colon lamina propria leukocytes (LPL), together with ATAC-seq, ChIP-seq and RNA-seq from colon LPL and sorted CD4+ T from colon lamina propria during oral infection with the pathobiont Helicobacter hepaticus we show that Blimp-1 and c-Maf both positively regulate Il10 gene expression, but also cooperate to negatively regulate a large network of proinflammatory effector genes in T cells. While T cell-specific deletion of either Prdm1, Maf or both transcription factors did not result in overt inflammation of the colon at steady state, H. hepaticus infection resulted in mild/moderate colitis in both the Prdm1fl/flCd4Cre and Maffl/flCd4Cre single T cell-specific transcription factor deficient mice, while the double knockout Prdm1fl/flMaffl/flCd4Cre infected mice suffered severe colitis and showed a massive increase in T cell effector genes in colonic LPL. Blimp-1 and c-Maf cooperate to negatively regulate genes including Ifng, Csf2, Il23r, Stat4, Il18r1 and Il2rg, and deletion of both these transcription factors in T cells translated to an exacerbated pathogenic TH1, IFN-g+GM-CSF+ effector cell response. c-Maf, additionally negatively regulated Il17a expression which was elevated in the LPL as assessed by bulk RNA-seq and scRNA-Seq analysis in the H. hepaticus infected Maffl/flCd4Cre mice.

2024-02-18 | GSE197784 | GEO

A Pathobiont of the Microbiota Balances Host Colonization and Intestinal Inflammation

Project description:The mammalian gastrointestinal tract harbors a diverse microbiota residing in intimate contact with the host immune system. Though most associations are symbiotic or commensal, some resident bacteria (termed pathobionts) have the potential to induce disease in immunocompromised hosts. Type VI secretion systems (T6SSs) have recently emerged as a novel mechanism for forging microbial-host interactions during infection. We reveal here a unique protective role for the T6SS of Helicobacter hepaticus, a Gram-negative bacterium of the murine intestinal microbiota. The T6SS of H. hepaticus targets effector substrates to intestinal epithelial cells (IECs). Mutants in T6SSs display higher intracellular and cell-associated numbers upon incubation with IECs, and exhibit increased bacterial colonization of the gastrointestinal tract compared to wild-type bacteria. The T6SS accordingly directs an anti-inflammatory gene expression profile in IECs co-cultured with H. hepaticus. Remarkably, T6SS mutants induce an exacerbated pro-inflammatory response in an experimental model of colitis. CD4+ T cells isolated from T6SS mutant-colonized animals produce increased T-helper 17 (Th17) cytokines in response to IECs presenting H. hepaticus antigens. These data demonstrate that H. hepaticus intimately interacts with IECs and employs type VI secretion to establish a balanced host relationship by limiting microbial colonization and intestinal inflammation. We propose that altering host-bacterial equilibriums that lead to dysbiosis of the microbiota contributes to human disorders such as inflammatory bowel disease and colon cancer.

2010-06-01 | GSE20434 | GEO

Transcription profiling of mouse tumors from H. hepaticus infected animals vs. infected and control mice

Project description:We are investigating the transcriptional response of mice infected with Helicobacter hepaticus and links to liver cancer; We used microarrays to detail the global programme of gene expression underlying H. hepaticus induced liver cancer Experiment Overall Design: Mice with tumors were compared to mice with infection only (comparison 2) and mice infected were compared to control mice (comparison 1)

2008-06-17 | E-GEOD-9097 | biostudies-arrayexpress

Transcription profiling of mouse Male A/JCr mice were inoculated with H. hepaticus or vehicle at 4 weeks some underwent castration at 1 year

Project description:We are investigating hepatic transcriptional responses associated with castration and tumorigenic hepatitis induced by Helicobacter hepaticus infection in mature male A/JCr mice; Microarray data demonstrated a global loss of gender-dimorphic gene expression regulation in mice with chronic hepatitis and liver cancer Experiment Overall Design: Male A/JCr mice were inoculated with H. hepaticus or vehicle at 4 weeks; some underwent castration at 1 year, and all were necropsied at 21 months

2008-06-15 | E-GEOD-6632 | biostudies-arrayexpress

fliA regulon of Helicobacter hepaticus

Project description:Differential gene regulation in Helicobacter hepaticus between wild type bacteria and fliA mutant 3 biological experiments of both wild type and fliA mutant bacteria (1-3) and 2 replicates (dye flip) of each biological experiment

2009-07-14 | E-GEOD-12123 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data