Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene-metabolite network analysis revealed tissue specific accumulation of therapeutic metabolites in Mallotus japonicus

ABSTRACT: In this study, we performed LC-QTOF-MS-based metabolomics and RNA-seq based transcriptome analysis using seven tissues of M. japonicus.

ORGANISM(S): Mallotus japonicus

PROVIDER: GSE179338 | GEO | 2021/08/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene-Metabolite Network Analysis Revealed Tissue-Specific Accumulation of Therapeutic Metabolites in Mallotus japonicus.

Project description:Mallotus japonicus is a valuable traditional medicinal plant in East Asia for applications as a gastrointestinal drug. However, the molecular components involved in the biosynthesis of bioactive metabolites have not yet been explored, primarily due to a lack of omics resources. In this study, we established metabolome and transcriptome resources for M. japonicus to capture the diverse metabolite constituents and active transcripts involved in its biosynthesis and regulation. A combination of untargeted metabolite profiling with data-dependent metabolite fragmentation and metabolite annotation through manual curation and feature-based molecular networking established an overall metabospace of M. japonicus represented by 2129 metabolite features. M. japonicus de novo transcriptome assembly showed 96.9% transcriptome completeness, representing 226,250 active transcripts across seven tissues. We identified specialized metabolites biosynthesis in a tissue-specific manner, with a strong correlation between transcripts expression and metabolite accumulations in M. japonicus. The correlation- and network-based integration of metabolome and transcriptome datasets identified candidate genes involved in the biosynthesis of key specialized metabolites of M. japonicus. We further used phylogenetic analysis to identify 13 C-glycosyltransferases and 11 methyltransferases coding candidate genes involved in the biosynthesis of medicinally important bergenin. This study provides comprehensive, high-quality multi-omics resources to further investigate biological properties of specialized metabolites biosynthesis in M. japonicus.

| S-EPMC8396295 | biostudies-literature

Systems-based characterization of specialized metabolites biosynthesis in Cornus officinalis

Project description:In this study, we performed LC-QTOFMS-based metabolomics and RNA-seq based transcriptome analysis using seven tissues of C. officinalis.

2020-07-07 | GSE144198 | GEO

Gene-metabolite network analysis revealed tissue specific accumulation of therapeutic metabolites in Mallotus japonicus

Project description:Gene-metabolite network analysis revealed tissue specific accumulation of therapeutic metabolites in Mallotus japonicus

| PRJNA743303 | ENA

Dataset Lotus Japonicus root exudates

Project description:The present dataset is composed of Lotus japonicus root exudate samples under different nitrogen states: starved (no nitrogen), inorganic N (KNO3), symbiotic N (inoculation with M. loti), and inorganic/symbiotic (KNO3 + M. loti). The samples were analyzed by ultra-high-performance liquid chromatography (UHPLC) coupled to a quadrupole time-of-flight mass spectrometer (qToF MS, Bruker Compact) with electrospray ionization.

2023-05-22 | MSV000092000 | MassIVE

Constitutive over-expression of LjMyb14 in Lotus japonicus.

Project description:To identify the regulatory targets of the R2R3-Myb transcription factor, LjMyb14, the gene was constitutively over-expressed in Lotus japonicus under the Lotus ubiquitin promoter. The gene expression levels of three biological replicates of the Lotus japonicus (MG20) were averaged and compared to the the gene expression levels of three independent lines of Lotus japonicus japonicus constituitively over expressing LjMyb14 using the Lotus ubiquitin promoter.

2011-09-15 | E-GEOD-31739 | biostudies-arrayexpress

Methylome of Lotus japonicus accession 'Gifu'

Project description:Whole genome DNA methylation data for the the Lotus japonicus accession 'Gifu'

2016-12-01 | GSE73069 | GEO

Cellvibrio japonicus chitin secretome

Project description:Study of the secretome of Cellvibrio japonicus after growth on different chitins. Utilization of a novel plate method to enrich truly secreted proteins.

2016-05-18 | PXD002843 | Pride

Lotus japonicus-Alkaline

Project description:Lotus japonicus is a model legume broadly used to study transcriptome regulation under different stress conditions and microorganism interaction. Understanding how this model plant respond gainst alkaline stress will certainly help to develop more tolerant cultivars in economically important Lotus species as well as in other legumes. In order to uncover the most important response mechanisms activated during alkaline stress, we explored by microarray analysis the transcriptome regulation occurring in the phenotypically contrasting ecotypes MG-20 and Gifu B-129 of L. japonicus after 21 days of alkaline stress.

2014-03-25 | E-MTAB-2418 | biostudies-arrayexpress

Natural DNA methylation variation in Lotus japonicus

Project description:Natural DNA methylation variation was examined in a population of wild Lotus japonicus accessions from Japan

2017-06-01 | GSE78767 | GEO

Mus musculus

Project description:Metabolomics LC-MS dataset

| PRJNA811808 | ENA