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Cellvibrio japonicus chitin secretome

ABSTRACT: Study of the secretome of Cellvibrio japonicus after growth on different chitins. Utilization of a novel plate method to enrich truly secreted proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Cellvibrio Japonicus (strain Ueda107) (pseudomonas Fluorescens Subsp. Cellulosa)

SUBMITTER: Tina Rise Tuveng

LAB HEAD: Vincent G. H. Eijsink

PROVIDER: PXD002843 | Pride | 2016-05-18

REPOSITORIES: Pride

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			Action	DRS
	20141216_CJ_Pl_a_6d_1.raw	Raw
	20141216_CJ_Pl_a_6d_1_2.raw	Raw
	20141216_CJ_Pl_a_6d_2.raw	Raw
	20141216_CJ_Pl_a_6d_2_2.raw	Raw
	20141216_CJ_Pl_a_6d_3.raw	Raw

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Publications

Proteomic investigation of the secretome of Cellvibrio japonicus during growth on chitin.

Tuveng Tina Rise TR Arntzen Magnus Øverlie MØ Bengtsson Oskar O Gardner Jeffrey G JG Vaaje-Kolstad Gustav G Eijsink Vincent G H VG

Proteomics 20160530 13

Studies of the secretomes of microbes grown on insoluble substrates are important for the discovery of novel proteins involved in biomass conversion. However, data in literature and this study indicate that secretome samples tend to be contaminated with cytoplasmic proteins. We have examined the secretome of the Gram-negative soil bacterium Cellvibrio japonicus using a simple plate-based culturing technique that yields samples with high fractions (60-75%) of proteins that are predicted to be sec ...[more]

PMID: 27169553

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