Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mettl3 regulates late-stage retinogenesis

ABSTRACT: To study the function of Mettl3 during retinal development, we used Six3-Cre and Mettl3floxed mice to conditionally knock out Mettl3 from retinal progenitor cells. The mutant mice show defects in late-stage retinogenesis and structural and physiological homeostasis of the retina.

ORGANISM(S): Mus musculus

PROVIDER: GSE180813 | GEO | 2022/12/03

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Mettl3 regulates late retinogenesis

Project description:Mettl3 was conditionally knocked out from retinal progenitor cells. Metll3 deficient retinas exhibited disrupted cell cycle during late retinogenesis and abnormality in retinal architecture and physiology.

2022-12-02 | GSE181095 | GEO

Mettl3 regulates late-stage retinogenesis

Project description:Mettl3 regulates late-stage retinogenesis

| PRJNA749618 | ENA

Mettl3 regulates late retinogenesis

Project description:Mettl3 regulates late retinogenesis

| PRJNA750706 | ENA

Mettl3 regulates late-stage retinogenesis and mouse retina m6A epitranscriptome

Project description:Mettl3 regulates late-stage retinogenesis and mouse retina m6A epitranscriptome

| PRJNA749616 | ENA

Catalytic activity of nuclear METTL3 promotes functional m6A modifications that drive retinal development [eCLIP]

Project description:METTL3 catalyzes N6-methyladenosine (m6A) on mRNA, regulating RNA metabolism, but its role in early retinal development remains virtually unstudied. Using stem cell-derived 3D retinal organoids to model retinal progenitor cell (RPC) differentiation, we found that loss of METTL3 nuclear m6A enzymatic activity impairs formation of the Rx+ retinal anlage in vitro. Through dCas13b-FTO m6A engineering, we demonstrate that m6A modifications at the Six3 3'UTR control its stability. While Mettl3 loss altered histone modifications, direct METTL3 chromatin targets were not transcriptionally functional. Integration of transcriptome-wide m6A and protein-RNA mapping with a degron-based strategy revealed immediate effects of METTL3 degradation in RPCs, uncovering a regulatory interaction between METTL3 and its RNA target Ythdf1. We demonstrate uncoupling of chromatin accessibility from changes in retinal transcription and m6A modifications. In vivo studies of Mettl3-deficient RPCs showed altered cell cycle and impaired retinal ganglion cell generation. Our findings establish METTL3-dependent m6A modifications as an essential post-transcriptional layer that drives retinal development.

2025-01-21 | GSE287302 | GEO

Catalytic activity of nuclear METTL3 promotes functional m6A modifications that drive retinal development [Nanopore-seq]

2025-01-25 | GSE287489 | GEO

Catalytic activity of nuclear METTL3 promotes functional m6A modifications that drive retinal development [ATAC-seq]

2025-01-25 | GSE287487 | GEO

Catalytic activity of nuclear METTL3 promotes functional m6A modifications that drive retinal development [mRNA-seq]

2025-01-25 | GSE287484 | GEO

Catalytic activity of nuclear METTL3 promotes functional m6A modifications that drive retinal development [ChIP-seq]

2025-01-25 | GSE287482 | GEO

Catalytic activity of nuclear METTL3 promotes functional m6A modifications that drive retinal development [RNA-Seq]

2025-01-21 | GSE287253 | GEO