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Genome-wide maps of Ets21C and Pointed isoforms PntP1 and PntP2 binding sites in intestinal stem cells (ISCs) and transcriptome profiling of ISCs over-expressing Spitz, Ets21C, PntP1, or PntP2

ABSTRACT: Purpose: The ETS transcription factors Ets21C and Pnt are downstream effectors of EGFR signaling pathway regulating intestinal stem cell (ISC) proliferation. ISC-specific DNA binding mapping using DamID and mRNA expression profiling were performed to identify the role of Ets21C and Pnt downstream of EGFR signaling. Methods: For DamID, ISC were FACS sorted and their genomic DNA extracted, amplified, and sequenced. For RNA-Seq, ISC were FACS sorted and their total RNA extracted, amplified (only for Pnt samples), and sequenced. Conclusions: We found that Ets21C and Pnt have a role in driving both ISC proliferation and metabolic changes.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE181583 | GEO | 2021/10/01

REPOSITORIES: GEO

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