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Role of Setd2 in regulatory T cells [H3K36me3 ChIP-seq]

ABSTRACT: ChIP-sequencing for H3K36me3 analysis was performed on Tregs purified from the spleen and large intestine of naïve mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE182340 | GEO | 2022/10/14

REPOSITORIES: GEO

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Role of Setd2 in regulatory T cells

Project description:This SuperSeries is composed of the SubSeries listed below. To determine the molecular regulation of Tregs by Setd2, Spleen or large intestine Tregs (CD3+CD4+Foxp3-YFP+) were purified from littermate Foxp3Cre-YFPSetd2f/+ and Foxp3Cre-YFPSetd2f/f mice. Setd2-deficient and control Tregs were subjected to RNA-seq, RNA Polymerase II CUT&Tag or H3K27ac CUT&Tag analyses.

2022-10-14 | GSE182845 | GEO

Role of Setd2 in regulatory T cells [H3K27ac CUT&Tag]

Project description:To determine the molecular regulation of Tregs by Setd2, large intestine Tregs (CD3+CD4+Foxp3-YFP+) from Setd2-deficient and control Tregs were subjected to H3K27ac CUT&Tag analysis.

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Role of Setd2 in regulatory T cells [Pol II CUT&Tag]

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Role of Setd2 in regulatory T cells [H3K36me3 ChIP-seq]

Project description:Role of Setd2 in regulatory T cells [H3K36me3 ChIP-seq]

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Role of Setd2 in regulatory T cells [RNAseqTreg]

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Role of Adenosine 2A Receptors (A2AR) on regulatory T cells (Tregs)

Project description:The adenosine 2A receptor (A2AR) is expressed on regulatory T cells (Tregs), but the functional significance is currently unknown. We compared the gene expression between wild-type (WT) and A2AR knockout (KO) Tregs and between WT Tregs treated with vehicle or a selective A2AR agonist. FACS-sorted GFP positive Tregs from WT or A2AR KO FoxP3GFP mouse spleen and lymph nodes were incubated 18 hr with vehicle (DMSO), a separate set of WT Tregs were incubated with the selective A2AR agonist ATL1222 10 nM (Dogwood Pharmaceuticals, Inc.) for 18 hr prior to RNA isolation.

2012-05-31 | E-GEOD-34006 | biostudies-arrayexpress

Role of Setd2 on regulatory T cells [RNA-seq SP Treg]

Project description:To determine the mechanism of regulation of Tregs by Setd2, SPTregs (CD3+CD4+Foxp3-YFP+) from Setd2-deficient and control Tregs were subjected to RNA-seq analysis.

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Microarray expression analysis depicting MHCII-dependent features of FOXP3-expressing CD4 T regulatory cells

Project description:Analysis comparing K14 and MCHII+/- Tregs. The hypothesis was that the lack of MHCII+/- in the spleen and intestine would result in a large shift in K14 Treg gene expression away from an activated and effector phenotype. Results demonstrate how a lack of MHCII-antigen presentation in the small intestine, where Tregs exhibit an effector penotype, does not greatly reduce effector features of T regulatory cells in K14 mice.

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