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Profiling of mRNA transcriptomic changes using RNA-seq in postmortem ventral tegmental area (VTA) of subjects with alcohol use disorder

ABSTRACT: Purpose: The goal of this study to examine mRNA transcriptomic changes in reward-related brain regions of subjects with alcohol use disorder. Methods: Total RNAs were extracted from postmortem ventral tegmental area of 12 AUD and 12 control subjects. rRNA depletion RNA sequencing was performed and the sequence reads were processed using the bulk RNA-seq processing pipeline Pipeliner workflow (Federico et al. Front Genet 2019; 10, 614). AUD-associated mRNA transcriptomic changes were analyzed by the Limma-Voom method. Results: Differentially expressed mRNAs (absolute FC>2.0 & P<0.05) were identified in postmortem ventral tegmental area of subjects with alcohol use disorder (AUD). Chronic alcohol consumption may alter mRNA transcriptome profiles in reward-related brain regions, resulting in alcohol-induced neuroadaptations.

ORGANISM(S): Homo sapiens

PROVIDER: GSE182649 | GEO | 2021/10/13

REPOSITORIES: GEO

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