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Expression data form mouse partial hepatectomy in Kras mutant and Bap1 deleted / Kras mutant mice

ABSTRACT: BAP1 is a nuclear deubiquitinating enzyme in the ubiquitin carboxyterminal-hydrolase subfamily that is involved in chromatin remodeling. It has been demonstrated to function as a tumor suppressor gene in multiple human malignancies. Molecular studies have found recurrent somatic mutations in BAP1 in intrahepatic cholangiocarcinoma. We have characterized the role of BAP1 in ICC development through a combination of whole genome ChIP-on-chip and transcriptional profiling of the mouse hepatic tumors. In this dataset, we include the expression data obtained from dissected mouse livers containing tumors using a gain-of-function mutation in KRAS (G12D), as well as loss-of-function mutation in BAP1. These data are used to obtain genes that are differentially expressed in response to Bap1 loss.

ORGANISM(S): Mus musculus

PROVIDER: GSE183554 | GEO | 2021/11/29

REPOSITORIES: GEO

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Genome wide binding sites of BAP1, HCF1 and OGT

Project description:We report the genome wide binding sites of BAP1, HCF1 and OGT in bone marrow derived macrophages. De-ubiquitinating enzyme BAP1 is mutated in a hereditary cancer syndrome with increased risk of mesothelioma and uveal melanoma. Somatic BAP1 mutations occur in various malignancies. We show that mouse Bap1 gene deletion is lethal during embryogenesis, but systemic or hematopoietic-restricted deletion in adults recapitulates features of human myelodysplastic syndrome (MDS). Knockin mice expressing BAP1 with a 3xFlag tag revealed that BAP1 interacts with host cell factor–1 (HCF-1), O-linked N-acetylglucosamine transferase (OGT), and the polycomb group proteins ASXL1 and ASXL2 in vivo. OGT and HCF-1 levels were decreased by Bap1 deletion, indicating a critical role for BAP1 in stabilizing these epigenetic regulators. Human ASXL1 is mutated frequently in chronic myelomonocytic leukemia (CMML) so an ASXL/BAP1 complex may suppress CMML. A BAP1 catalytic mutation found in a MDS patient implies that BAP1 loss of function has similar consequences in mice and humans. For BAP1, bone marrow derived macrophages were used differentiated from bone marrow cells of BAP1-3X Flag Tagged KI mice we generated. For OGT and HCF1, bone marrow derived macrophages were used from BAP1 WT mice.

2012-11-07 | E-GEOD-40723 | biostudies-arrayexpress

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Probing the Tumour Suppressor Function of BAP1 in CRISPR-engineered Human Liver Organoids

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Expression data form mouse partial hepatectomy in Kras mutant and Bap1 deleted / Kras mutant mice

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