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Transcriptome analysis of immortalized OPLL ligament cells

ABSTRACT: Ossification of the posterior longitudinal ligament (OPLL) is formed by heterogeneous ossification of posterior longitudinal ligament. The patho-mechanism of OPLL is still largely unknown. For the rarity of the primary cell samples, here we immortalized the OPLL patient's posterior longitudinal ligament primary cells and normal patient's posterior longitudinal ligament primary cells. The transcriptome of the cells were compared and we found that the osteogenic ability of the OPLL ligament cells were inherited in the immortalized cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE183752 | GEO | 2024/12/31

REPOSITORIES: GEO

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Transcriptome and Micronome analysis of OPLL

Project description:Ossification of the posterior longitudinal ligament (OPLL) is formed by heterogeneous ossification of posterior longitudinal ligament. The patho-mechanism of OPLL is still largely unknown. MicroRNAs are small nucleatides that function as regulators of gene expression in almost any biological process. However, few microRNAs are reported to have a role in the pathological process of OPLL. Therefore, we performed high-throughput microRNA sequencing and transcriptome sequencing of primary OPLL and PLL cells in order to decipher the interacting network of microRNAs in OPLL. MRNA and microRNA profiles were done using primary culture cells of human ossification of the posterior longitudinal ligament (OPLL) tissue and normal posterior longitudinal ligament (PLL) tissue.

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