Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Changes of transcriptome in Alfalfa (Medicago sativa L.) under selenium treatment

ABSTRACT: We studied the application of transcriptome technology in alfalfa selenium treatment. After spraying sodium selenite on the leaves, the process of selenium absorption and assimilation of alfalfa is unknown. The time point of transcriptome determination was determined by measuring the change of selenium content. Our results showed that 12 h was the key point of the change of selenium content in alfalfa, that is, the selenium content increased continuously before 12 h, decreased gradually after 12 h, and remained stable after 48 h. Transcriptome sequencing showed that phosphorus transporter and endocytosis related genes may be involved in selenium absorption at 12 h compared with 0 H. 12-48 h, some thiometabolic pathways may be involved in selenium metabolism and ubiquitination pathway, which may be the detoxification pathway of selenoprotein.

ORGANISM(S): Medicago sativa

PROVIDER: GSE186868 | GEO | 2024/10/27

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptomic analysis of Alfalfa response to low selenium concentration

Project description:We studied the application of transcriptome technology in alfalfa selenium (Se) treatment. Alfalfa had different states after different concentrations of Se treatment. It shows that lower concentration promoted growth and higher concentration produced toxicity. The positive regulatory effects of moderate Se (100 mg / kg) on alfalfa was determined through preliminary experiments, and the gene expression of Alfalfa under this treatment was further analyzed by transcriptome.

2023-08-09 | GSE192349 | GEO

Proteomic and transcriptomic analysis of selenium utilization in Methanococcus maripaludis

Project description:Methanococcus maripaludis utilizes selenocysteine-(Sec-) containing proteins (selenoproteins), mostly active in the organism’s primary energy metabolism, methanogenesis. Under selenium depletion, M. maripaludis employs a set of enzymes containing cysteine (Cys) instead of Sec. The genes coding for these Sec-/Cys-containing isoforms are the only genes known expression of which is influenced by the selenium status of the cell. Using quantitative proteomics and transcriptomics approx. 7% and 12%, respectively, of all genes/proteins were differentially expressed/synthesized in response to the selenium supply. Some of the genes identified involve methanogenesis, nitrogenase functions, and putative transporters. An increase of transcript abundance for putative transporters under selenium-depleted conditions indicated the organism’s effort to tap into alternative sources of selenium. Selenium sources M. maripaludis is known to utilize are selenite and dimethylselenide. To expand this list, a selenium responsive reporter strain was assessed with nine other, environmentally relevant selenium species. While some had a similar biological window as selenite, others were effectively utilized at lower concentrations. Conversely, selenate and seleno-amino acids were only utilized at unphysiologically high concentrations and two compounds were not utilized at all. To address the role of the selenium-regulated putative transporters in selenium transport, M. maripaludis mutant strains lacking one or two of the putative transporters were tested for the capability to utilize the different selenium species. Of the five putative transporters analyzed by loss-of-function mutagenesis, none appeared to be absolutely required for utilizing any of the selenium species tested, indicating they have redundant and/or overlapping specificities, or are not dedicated selenium transporters.

2024-03-27 | PXD047095 | Pride

Transcriptome of Pseudomonas putida KT2440 in response to selenite

Project description:The bacterium Pseudomonas putida KT2440 has the ability to reduce selenite forming nanoparticles of elemental selenium. This is the transcriptome of the organism when cultured in the presence of selenite.

2022-10-01 | GSE214391 | GEO

Transcriptomic profiling of S. cerevisiae wild type and aft2 mutant in response to exposure to selenite

Project description:The yeast S. cerevisiae does not require selenium for growth, so it is an excellent model to investigate the toxicity of this element without interference from its requirement as growth factor as occurs in animal cells. Exposure to selenite interferes with the yeast iron metabolism. Aft2 is a transcription factor related (with Aft1) to maintenance of iron homeostasis. We have found that aft2 cells are highly sensitive to selenite (a common environmental source for selenium). In these experiments we investigate the transcriptional response to a sub-lethal dose of selenite in wild type and aft2 cells. Our results show that the aft2 mutation strongly potentiates the transcriptional response to selenite, showing induction of many genes related to the responses to oxidative stress and DNA damage. Wild type (W303-1A) background and its MML1086 derivative (Daft2) were exposed to 1 mm selenite and samples taken at time 0, 1, 3 and 5 h of selenite treatment. Two biological replicates were prepared for each time-point. Therefore, there are 8 samples per replicate (4 samples for WT and 4 samples for aft2 cells). The WT strain is used here as reference.

2015-12-31 | E-GEOD-70835 | biostudies-arrayexpress

Transcriptomic profiling of S. cerevisiae wild type and aft2 mutant in response to exposure to selenite

2015-12-31 | GSE70835 | GEO

MtIFS1-expressing alfalfa

Project description:We used an M. truncatula 16K oligonucleotide array to profile gene expression in the leaves of transgenic alfalfa plants expressing Medicago truncatula isoflavone synthase 1 (MtIFS1). RNA purified from leaves of MtIFS1-expressing lines C22 and B20 and vector control line VC11 was used to generate Cy5-labeled cDNA. RNA purified from a second vector control line, VB2, was used to generate Cy3-labeled reference cDNA. Three independently propagated cuttings of each line were used and a total of nine hybridizations were performed. Our results indicated that MtIFS1-expression does not significantly alter global gene expression in the leaves. Keywords = Medicago Keywords = isoflavone synthase Keywords = alfalfa Keywords: other

2005-07-20 | GSE2546 | GEO

Small RNA-seq for alfalfa roots under alkaline stress

Project description:Abstract: In order to understand the expression patterns of miRNAs in alfalfa under alkali stress, small RNA sequencing was performed on alfalfa roots at different time points under alkali stress, and miRNAs were identified and analyzed.

2023-05-08 | GSE231697 | GEO

RNAseq of the effect of selenite and coated selenium nanoparticles against spheroids of SKOV-3 and OVCAR-3 ovarian cancer cell lines

Project description:Analysis of transcriptome changes in SKOV-3 and OVCAR-3 spheroids following selenite or coated selenium nanoparticles treatment at sublethal doses for 24 hrs

2023-02-24 | GSE149397 | GEO

Transcriptomic analysis of Alfalfa response to low selenium concentration

Project description:Transcriptomic analysis of Alfalfa response to low selenium concentration

| PRJNA791223 | ENA

ALFALFA

Project description:ALFALFA Raw sequence reads

| PRJNA884854 | ENA