Transcriptomics

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Circular RNA signatures of human healing and non-healing wounds


ABSTRACT: In an RNAseq analysis, we have identified the circular RNAs cCHST15 and cTNFRSF21 with high levels in venous ulcers compared to acute wounds or skin. The biological function of these two circular RNAs in human epidermal keratinocytes during wound repair has not been studied. To study the genes regulated by cCHST15 or cTNFRSF21 , we transfected siRNA targeting the diagnostic junctions of either cCHST15 or cTNFRSF21 into human primary epidermal keratinocytes to knockdown cCHST15 or cTNFRSF21 expression, respectively. We performed a global transcriptome analysis of keratinocytes upon circRNA knockdown using Affymetrix arrays.

ORGANISM(S): Homo sapiens

PROVIDER: GSE188939 | GEO | 2022/03/06

REPOSITORIES: GEO

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