Transcriptomics

Dataset Information

0

Rapid and robust directed differentiation of mouse epiblast stem cells into definitive endoderm and forebrain organoids I


ABSTRACT: Mouse is the pre-eminent model system for studying genetic regulation of embryonic development. Directed differentiation of pluripotent stem cells (PSCs) has emerged as a powerful complement to in vivo studies of development, allowing for examination of developmental mechanisms at the molecular and cellular level. However, the limited availability of high-quality protocols for directed differentiation of mouse PSCs into defined lineages and organoids has prevented the widespread application of mouse PSC directed differentiation models. Here, we examine the potential of mouse epiblast stem cells (EpiSCs) cultured in media containing Wnt pathway inhibitors (primed ground state conditions) as a starting point for directed differentiation. First, we systematically optimized conditions to generate definitive endoderm that are significantly faster and more efficient than current state-of-the-art protocols for mouse PSCs. Second, we developed a robust protocol for generation of forebrain-patterned organoids. These new models can complement and empower in vivo studies in the mouse and can inform human PSC-based studies of development, which cannot readily be compared to analogous stages in vivo.

ORGANISM(S): Mus musculus

PROVIDER: GSE189869 | GEO | 2021/12/31

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2021-12-31 | GSE189870 | GEO
2013-04-15 | E-GEOD-42139 | biostudies-arrayexpress
2021-12-05 | GSE178263 | GEO
2024-07-21 | GSE272490 | GEO
2013-04-15 | GSE42139 | GEO
2013-02-23 | E-GEOD-30868 | biostudies-arrayexpress
2014-10-03 | E-GEOD-43770 | biostudies-arrayexpress
2013-01-17 | GSE43496 | GEO
2019-12-17 | GSE129104 | GEO
2024-07-31 | GSE237774 | GEO