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HSP70 binds RNA and regulates transcription by RNA Polymerase III [RNA-seq]

ABSTRACT: We examined the RNA binding ability of the 70-kDa heat shock protein (HSP70) family member HSPA1A and identified the RNA species bound to HSP70 in vivo. We used a UV-crosslinkiung and immunoprecipitation based approach named FLASH-seq (Aktaş et al., 2017) using 3xFLAG-Histidine-Biotin tagged ectopically expressed protein in HEK293T cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE191244 | GEO | 2024/01/17

REPOSITORIES: GEO

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HSP70 binds RNA and regulates transcription by RNA Polymerase III [ChIP-seq]

Project description:We examined the RNA binding ability of the 70-kDa heat shock protein (HSP70) family member HSPA1A and identified the RNA species bound to HSP70 in vivo. We used a UV-crosslinkiung and immunoprecipitation based approach named FLASH-seq (Aktaş et al., 2017) using 3xFLAG-Histidine-Biotin tagged ectopically expressed protein in HEK293T cells.

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HSP70 binds RNA and regulates transcription by RNA Polymerase III [FLASH-seq]

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HSP70 binds RNA and regulates transcription by RNA Polymerase III [FLASH-seq]

Project description:HSP70 binds RNA and regulates transcription by RNA Polymerase III [FLASH-seq]

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HSP70 binds RNA and regulates transcription by RNA Polymerase III [RNA-seq]

Project description:HSP70 binds RNA and regulates transcription by RNA Polymerase III [RNA-seq]

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