Proteomics

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MLF2-MBP purification (Table S4, Prophet et al.)

ABSTRACT: MLF2 was purified from mammalian Expi293 cells as a fusion with MBP. As MLF2 associates tightly with HSP70/HSPA1A, this resulted in a stoichiometric co-purification with HSP70 members. To identify which ones, we subjected the whole purification or a gel-extracted band around 70 kDa to mass spectrometry.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Christian Schlieker  

PROVIDER: MSV000090188 | MassIVE | Tue Aug 23 07:12:00 BST 2022

REPOSITORIES: MassIVE

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