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CRSP: Comparative RNA-seq pipeline for species lacking both of sequenced genomes and reference transcripts

ABSTRACT: RNA sequencing (RNA-seq) has been a widely used high-throughput method to characterize transcriptomic dynamics spatiotemporally. However, typical RNA-seq data analysis pipelines depend on either a sequenced genome or reference transcripts. This constriction makes the use of RNA-seq for species lacking both of sequenced genomes and reference transcripts challenging. To solve this problem, we developed CRSP, an RNA-seq pipeline integrating multiple comparative species strategy but not depending on a specific sequenced genome or reference transcripts. Benchmarking suggests the CRSP tool can achieve high accuracy to quantify gene expression levels.

ORGANISM(S): Homo sapiens

PROVIDER: GSE194237 | GEO | 2023/08/10

REPOSITORIES: GEO

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