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Higher intelligence is genetically linked with longevity

ABSTRACT: We report the application of mRNA sequencing technology for identifying that the genetic linkage of higher intelligence with longevity in Drosophilla melanogaster. a transcriptome analysis was performed by sequencing the total mRNA. The differentially expressed gene analysis by a negative binomial test showed that a subset of genes displayed remarkably altered expression where 56, 152, and 26 genes were significantly upregulated (adjusted P value <0.01), and 30, 397, and 10 genes were downregulated (adjusted P value < 0.01) in INT compared to F0, NINT compared to F0, and INT compared to NINT each. The functions of the differentially expressed genes were analyzed at high levels by using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment program. Compared to F0, the pathways of ribosome and autophagy were up-regulated in INT while the pathways related to metabolisms were down-regulated. Meanwhile, the pathways associated with genome stability and immune reaction was upregulated and the pathway of oxidative phosphorylation responsible for ATP generation was down regulated in NINT. The KEGG comparison of INT with NINT, which eliminates genes associated with the environmental factors during the selection process, visualized more precise pathways associated with higher intelligence and longevity. The pathways related to genome stability (Fanconi anemia pathway, Mismatch repair, DNA replication, Homologous recombination, and Nucleotide excision repair) and immune reaction (Toll and lmd signaling pathway) were up-regulated in NINT, and these pathways were down-regulated in INT.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE196981 | GEO | 2023/02/17

REPOSITORIES: GEO

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Project description:We investigated the nutritional effects on gene expression in sperm cells of F0 boars from a three generation Large White pig feeding experiment. A group of experimental (E) F0 boars were fed a standard diet supplemented with high amounts of methylating micronutrients whereas a control (C) group of F0 boars received a standard diet. These differentially fed F0 boars sired F1 boars which then sired 60 F2 pigs which were investigated in a previous study. The aim of this study was to investigate if the nutrition affects gene expression in sperm cells of differentially fed boars and thus carry information in the form of RNA molecules to the next generation. Four RNA samples from sperm cells of these differentially fed boars were analyzed by RNA-Seq methodology. We found no differential RNA expression in sperm cells of the two groups based on the adjusted P-value > 0.05. Nevertheless, we performed a pathway analysis with 105 genes that differed in gene expression on the level of nominal P-value < 0.05 between the two diet groups. We found a significant number of these differentially expressed genes were enriched for the pathway maps of bacterial infections in cystic fibrosis (CF) airways, glycolysis and gluconeogenesis p.3 and cell cycle_Initiation of mitosis. The GO processes including a significant portion of differentially expressed genes were viral transcription and viral genome expression, viral infectious cycle, cellular protein localization, cellular macromolecule localization, nuclear-transcribed mRNA catabolic process and nonsense-mediated decay. In summary, the results of the pathway analysis are also inconclusive and it is concluded that RNA expression in sperm cells is not significantly affected by extensive supplementation of methylating micronutrients. Consequently, RNA molecules could not be established as epigenetic marks in this feeding experiment. Gene expression in sperm cells from differentially fed F0 boars was measured. F0 boars received either a standard diet or a standard diet supplemented with methylating micronutrients. These boars were used to study transgenerational epigenetic inheritance in a three generation pig pedigree. Therefore it was of interest if the diet affects gene expression in sperm cells which could then be transmitted to next generations.