Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA314575 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:General intelligence (g) is a common core shared by cognitive tasks. To study epigenetic regulation of “g”, 41 inbred mice raised under similar environmental conditions were subjected to five behavioral tests. We used principal component analysis to analyze 11 parameters that measure performance and reflect cognition of mice. Microarray gene expression analysis by using hippocampal RNAs of the 5, 12, and 8 mice consistently classified as having high, medium, and low cognition, respectively, by PC1 score and IQ score identified 17 genes that showed at least 1.3-fold difference in expression between mice with high and low cognition (FDR<0.30). Two of these genes were down-regulated and 15 of these genes were up-regulated in the high cognitive mice. Subsequent pathway-based analysis further identified gene-sets (GS) enriched in high cognitive mice and GS enriched in low cognitive mice. A number of genes implicated in our study have been previously associated with cognitive impairments. Our findings provide insights into genes and pathways regulated by epigenetic mechanisms in determining “g” and may shed lights on various disorders that affect cognitive function.
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Project description:We report the application of mRNA sequencing technology for identifying that the genetic linkage of higher intelligence with longevity in Drosophilla melanogaster. a transcriptome analysis was performed by sequencing the total mRNA. The differentially expressed gene analysis by a negative binomial test showed that a subset of genes displayed remarkably altered expression where 56, 152, and 26 genes were significantly upregulated (adjusted P value <0.01), and 30, 397, and 10 genes were downregulated (adjusted P value < 0.01) in INT compared to F0, NINT compared to F0, and INT compared to NINT each. The functions of the differentially expressed genes were analyzed at high levels by using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment program. Compared to F0, the pathways of ribosome and autophagy were up-regulated in INT while the pathways related to metabolisms were down-regulated. Meanwhile, the pathways associated with genome stability and immune reaction was upregulated and the pathway of oxidative phosphorylation responsible for ATP generation was down regulated in NINT. The KEGG comparison of INT with NINT, which eliminates genes associated with the environmental factors during the selection process, visualized more precise pathways associated with higher intelligence and longevity. The pathways related to genome stability (Fanconi anemia pathway, Mismatch repair, DNA replication, Homologous recombination, and Nucleotide excision repair) and immune reaction (Toll and lmd signaling pathway) were up-regulated in NINT, and these pathways were down-regulated in INT.
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