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Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration [MSC]

ABSTRACT: We reported high-density encapsulation of MSCs in Matrigel beads, augmenting MSCs function, including increasing MSCs proliferation, migration, and extracellular vesicles (EVs) production via elevated PI3K-AKT pathway activity.

ORGANISM(S): Homo sapiens

PROVIDER: GSE197355 | GEO | 2022/02/28

REPOSITORIES: GEO

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Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration [skeletal muscle]

Project description:We reported high-density encapsulation of MSCs in Matrigel beads, distributed in sparse pattern, augmenting mouse volumetric skeletal muscle tissue with reduced fibrosis.

2022-02-28 | GSE197356 | GEO

Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-02-28 | GSE197358 | GEO

Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration [MSC]

Project description:Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration [MSC]

| PRJNA810031 | ENA

Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration

Project description:Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration

| PRJNA810023 | ENA

Bio-printing cell-laden Matrigel-agarose constructs.

Project description:3D printing of biological architectures that mimic the structural and functional features of in vivo tissues is of great interest in tissue engineering and the development of transplantable organ constructs. Printable bio-inks that are compatible with cellular activities play critical roles in the process of 3D bio-printing. Although a variety of hydrogels have been used as bio-inks for 3D bio-printing, they inherit poor mechanical properties and/or the lack of essential protein components that compromise their performance. Here, a hybrid Matrigel-agarose hydrogel system has been demonstrated that possesses both desired rheological properties for bio-printing and biocompatibility for long-term (11 days) cell culture. The agarose component in the hybrid hydrogel system enables the maintenance of 3D-printed structures, whereas Matrigel provides essential microenvironments for cell growth. When human intestinal epithelial HCT116 cells are encapsulated in the printed Matrigel-agarose constructs, high cell viability and proper cell spreading morphology are observed. Given that Matrigel is used extensively for 3D cell culturing, the developed 3D-printable Matrigel-agarose system will open a new way to construct Matrigel-based 3D constructs for cell culture and tissue engineering.

| S-EPMC5603305 | biostudies-literature

Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration [skeletal muscle]

Project description:Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration [skeletal muscle]

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Bead-jet printing enabled sparse mesenchymal stem cell patterning augments skeletal muscle and hair follicle regeneration.

Project description:Transplantation of mesenchymal stem cells (MSCs) holds promise to repair severe traumatic injuries. However, current transplantation practices limit the potential of this technique, either by losing the viable MSCs or reducing the performance of resident MSCs. Herein, we design a "bead-jet" printer, specialized for high-throughput intra-operative formulation and printing of MSCs-laden Matrigel beads. We show that high-density encapsulation of MSCs in Matrigel beads is able to augment MSC function, increasing MSC proliferation, migration, and extracellular vesicle production, compared with low-density bead or high-density bulk encapsulation of the equivalent number of MSCs. We find that the high-density MSCs-laden beads in sparse patterns demonstrate significantly improved therapeutic performance, by regenerating skeletal muscles approaching native-like cell density with reduced fibrosis, and regenerating skin with hair follicle growth and increased dermis thickness. MSC proliferation within 1-week post-transplantation and differentiation at 3 - 4 weeks post-transplantation are suggested to contribute therapy augmentation. We expect this "bead-jet" printing system to strengthen the potential of MSC transplantation.

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Mesenchymal stem cell-laden matrigel microspheres printing enables large skin wound regeneration

Project description:High cell density Matrigel beads (HDB) were printinto onto large skin wounds (10 mm and 15 mm). Compared with acellular beads printing, HDB group could achieve more efficient injury regeneration.

2022-10-12 | GSE214868 | GEO

Mesenchymal stem cell-laden matrigel microspheres printing enables large skin wound regeneration

Project description:Mesenchymal stem cell-laden matrigel microspheres printing enables large skin wound regeneration

| PRJNA887427 | ENA

Jet-Printing Microfluidic Devices on Demand.

Project description:There is an unmet demand for microfluidics in biomedicine. This paper describes contactless fabrication of microfluidic circuits on standard Petri dishes using just a dispensing needle, syringe pump, three-way traverse, cell-culture media, and an immiscible fluorocarbon (FC40). A submerged microjet of FC40 is projected through FC40 and media onto the bottom of a dish, where it washes media away to leave liquid fluorocarbon walls pinned to the substrate by interfacial forces. Such fluid walls can be built into almost any imaginable 2D circuit in minutes, which is exploited to clone cells in a way that beats the Poisson limit, subculture adherent cells, and feed arrays of cells continuously for a week. This general method should have wide application in biomedicine.

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