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Mesenchymal stem cell-laden matrigel microspheres printing enables large skin wound regeneration

ABSTRACT: High cell density Matrigel beads (HDB) were printinto onto large skin wounds (10 mm and 15 mm). Compared with acellular beads printing, HDB group could achieve more efficient injury regeneration.

ORGANISM(S): Mus musculus

PROVIDER: GSE214868 | GEO | 2022/10/12

REPOSITORIES: GEO

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Comparison of small RNA Profiles of extracellular vesicles (EVs) derived from three mesenchymal stem cell-laden Matrigel composites

Project description:To investigate the difference of small RNA profiles of EVs derived from mesenchymal stem cell-laden Matrigel composites: high cell density beads (HDB) , low cell density beads (LDB) , and high cell density bulk ge (HDG)

2022-10-17 | GSE215294 | GEO

Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration [skeletal muscle]

Project description:We reported high-density encapsulation of MSCs in Matrigel beads, distributed in sparse pattern, augmenting mouse volumetric skeletal muscle tissue with reduced fibrosis.

2022-02-28 | GSE197356 | GEO

Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration [MSC]

Project description:We reported high-density encapsulation of MSCs in Matrigel beads, augmenting MSCs function, including increasing MSCs proliferation, migration, and extracellular vesicles (EVs) production via elevated PI3K-AKT pathway activity.

2022-02-28 | GSE197355 | GEO

Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration

Project description:Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration

| PRJNA810023 | ENA

Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration [MSC]

Project description:Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration [MSC]

| PRJNA810031 | ENA

Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration [skeletal muscle]

Project description:Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration [skeletal muscle]

| PRJNA810028 | ENA

Bio-printing cell-laden Matrigel-agarose constructs.

Project description:3D printing of biological architectures that mimic the structural and functional features of in vivo tissues is of great interest in tissue engineering and the development of transplantable organ constructs. Printable bio-inks that are compatible with cellular activities play critical roles in the process of 3D bio-printing. Although a variety of hydrogels have been used as bio-inks for 3D bio-printing, they inherit poor mechanical properties and/or the lack of essential protein components that compromise their performance. Here, a hybrid Matrigel-agarose hydrogel system has been demonstrated that possesses both desired rheological properties for bio-printing and biocompatibility for long-term (11 days) cell culture. The agarose component in the hybrid hydrogel system enables the maintenance of 3D-printed structures, whereas Matrigel provides essential microenvironments for cell growth. When human intestinal epithelial HCT116 cells are encapsulated in the printed Matrigel-agarose constructs, high cell viability and proper cell spreading morphology are observed. Given that Matrigel is used extensively for 3D cell culturing, the developed 3D-printable Matrigel-agarose system will open a new way to construct Matrigel-based 3D constructs for cell culture and tissue engineering.

| S-EPMC5603305 | biostudies-other

Scalable Microfluidics for Single Cell RNA Printing and Sequencing

2015-05-21 | GSE66357 | GEO

Bead-Jet printing of high-density mesenchymal stem cell-laden Matrigel beads augments skeletal muscle and hair follicle regeneration

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-02-28 | GSE197358 | GEO

Scalable Microfluidics for Single Cell RNA Printing and Sequencing

Project description:Single cell transcriptomics has emerged as a powerful approach to dissecting phenotypic heterogeneity in complex, unsynchronized cellular populations. However, many important biological questions demand quantitative analysis of large numbers of individual cells. Hence, new tools are urgently needed for efficient, inexpensive, and parallel manipulation of RNA from individual cells. We report a simple microfluidic platform for trapping single cell lysates in sealed, picoliter microwells capable of “printing” RNA on glass or capturing RNA on polymer beads. To demonstrate the utility of our system for single cell transcriptomics, we developed a highly scalable technology for genome-wide, single cell RNA-Seq. The current implementation of our device is pipette-operated, profiles hundreds of individual cells in parallel with library preparation costs of ~$0.10-$0.20/cell, and includes five lanes for simultaneous experiments. We anticipate that this system will ultimately serve as a general platform for large-scale single cell transcriptomics, compatible with both imaging and sequencing readouts.!Series_type = Expression profiling by high throughput sequencing A microfluidic device that pairs sequence-barcoded mRNA capture beads with individual cells was used to barcode cDNA from individual cells which was then pre-amplified by in vitro transcription in a pool and converted into an Illumina RNA-Seq library. Libraries were generated from ~600 individual cells in parallel and extensive analysis was done on 396 cells from the U87 and MCF10a cell lines and from ~500 individual cells with extensive analysis on 247 cells from the U87 and WI-38 cell lines. Sequencing was done on the 3'-end of the transcript molecules. The first read contains cell-identifying barcodes that were present on the capture bead and the second read contains a unique molecular identifier (UMI) barcode, a lane-identifying barcode, and then the sequence of the transcript.

2015-05-21 | E-GEOD-66357 | biostudies-arrayexpress

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