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RNA-seq of THP-1 cells stimulated with CpG-c and Hydroxychloroquine intervention

ABSTRACT: Purpose: To investigate the inhibition of inflammatoty cytokines of Hydroxychloroquine to phagocytic THP-1 cells. Methods: we established a cell model of hemophagocytosis by simulating THP-1 cells with CpG-c for 24h in vitro to produce macrophages, and then coculture the cells with human RBCs. RNA sequencing analysis of THP-1 cells were performed to find Gene changed with CpG-c stimulation and Hydroxychloroquine intervention. Results:CpG-c sitimulation significantly altered gene expression of THP-1 cells. Cytokine genes were upregulated, especially a significant increase in IL-6 level from scratch. HCQ significantly inhibited the expression of inflammatory cytokines and decreased IL-6 gene level to baseline. Conclusions:Hydroxychloroquine can inhibite cytokine genes, especially IL-6 Gene level of THP-1 cells caused by CpG-c sitimulation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE198290 | GEO | 2022/07/01

REPOSITORIES: GEO

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RNA-seq of THP-1 cells stimulated with CpG-c and Hydroxychloroquine intervention

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