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Engineering bone-forming callus-organoid implants in a xenogeneic free differentiation medium

ABSTRACT: The field of tissue engineering aspires to provide clinically relevant solutions for patients through the integration of developmental engineering principles with a bottom up manufacturing approach. However, manufacturing of cell based advanced therapy medicinal products is hampered by protocol complexity, lack of non-invasive critical quality controls, and dependency on animal-derived components for tissue differentiation. We investigate a serum-free, chemically defined, xeno- and lipid-free chondrogenic differentiation medium to generate bone-forming callus organoids. Our results show an increase in microtissue homogeneity during prolonged differentiation and a high quality of in vivo bone forming organoids. The low protein content of the culture media potentially allows for monitoring of relevant secreted biomarkers as (critical) quality attributes . Together, we envisage that this xeno- and lipid-free chondrogenic medium is compatible with industrial scale-up and automation, while facilitating the implementation of non-invasive imaging and use of quality control parameters based on secreted biomarkers.

ORGANISM(S): Homo sapiens

PROVIDER: GSE198914 | GEO | 2022/08/25

REPOSITORIES: GEO

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