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MRNA microarray of aortae from 10-week male Fbn1mgR/mgR and wild-type mice [10-week_mRNA]

ABSTRACT: Thoracic aortic aneurysms (TAA) in Marfan syndrome, caused by fibrillin-1 mutations, are characterized by elevated cytokines and fragmentated elastic laminae in the aortic wall. This study explored whether and how specific fibrillin-1-regulated miRNAs mediate inflammatory cytokine expression and elastic laminae degradation in TAA. miRNA expression profiling at early and late TAA stages using a severe Marfan mouse model (Fbn1mgR/mgR) revealed a spectrum of differentially regulated miRNAs. Bioinformatic analyses predicted the involvement of these miRNAs in inflammatory and extracellular matrix related pathways. Complementing mRNA microarray demonstrated the upregulation of multiple pro-inflammatory cytokines and matrix metalloproteases. Correlation analyses between the altered miRNAs and mRNAs present miR-122, the most downregulated miRNAs in the TAA tissue of Fbn1mgR/mgR, to be a core regulatory miRNA to pro-inflammatory cytokines, Ccl2 and Il1b, which were upregulated in the TAA tissues. A total of 471 mRNAs were up-regulated (green) and 253 downregulated (red) more than 2-fold (p<0.05) in the aneurysmal versus the wild-type tissues.

ORGANISM(S): Mus musculus

PROVIDER: GSE199284 | GEO | 2022/05/21

REPOSITORIES: GEO

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microRNA microarray of aortae from 10-week male Fbn1mgR/mgR and wild-type mice [10-week_miRNA]

Project description:Thoracic aortic aneurysms (TAA) in Marfan syndrome, caused by fibrillin-1 mutations, are characterized by elevated cytokines and fragmentated elastic laminae in the aortic wall. This study explored whether and how specific fibrillin-1-regulated miRNAs mediate inflammatory cytokine expression and elastic laminae degradation in TAA. miRNA expression profiling at early and late TAA stages using a severe Marfan mouse model (Fbn1mgR/mgR) revealed a spectrum of differentially regulated miRNAs. Bioinformatic analyses predicted the involvement of these miRNAs in inflammatory and extracellular matrix related pathways. Complementing mRNA microarray demonstrated the upregulation of multiple pro-inflammatory cytokines and matrix metalloproteases. Correlation analyses between the altered miRNAs and mRNAs present miR-122, the most downregulated miRNAs in the TAA tissue of Fbn1mgR/mgR, to be a core regulatory miRNA to pro-inflammatory cytokines, Ccl2 and Il1b, which were upregulated in the TAA tissues. At 10 weeks, the number of differentially regulated miRNAs (>2-fold) increased to 129 with 5 down- and 124 upregulated miRNAs

2022-05-21 | GSE199283 | GEO

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