Proteomics

Dataset Information

0

Proteomic analysis of primary human microvascular endothelial cells (HMVECs) stimulated for 24 h with either 50 ï�g/mL recombinant Fibrillin-1 C-terminal fragment or 25 ng/ml VEGF-A compared to unstimulated cells.


ABSTRACT: Mutations in the fibrillin-1 gene result in cardiovascular, ocular, and skeletal abnormalities in Marfan syndrome. Here we show that a fibrillin-1 mutation also alters the neovessel formation. In the mouse retina vascularization model, fibrillin-1 is detected at the basement membrane underlying the angiogenic front and around arterioles. In Fbn1C1041G/+ mice, a model of Marfan disease, the microvasculature is altered at these sites. At the front, endothelial tip-cell sprouting and branching are decreased, and we show that mutant fibrillin-1 delays angiogenesis by affecting Notch signalling. Supplying the growing vasculature of Fbn1C1041G/+ mice with a C-terminal fragment of fibrillin-1 corrects all defects. In vitro, the C-terminal fragment of fibrillin-1 also displays pro-angiogenic activities on microvascular endothelial cell. Our data establish that fibrillin-1 performs essential functions in microvessel formation and integrity and that mutant fibrillin-1-induced defects can be rescued pharmacologically.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Microvascular Endothelial Cell

SUBMITTER: Dupuy Jean-William  

LAB HEAD: Elisabeth Genot

PROVIDER: PXD029834 | Pride | 2023-05-31

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Link_between_files.xlsx Xlsx
UP000005640_200903_Homo_sapiens.fasta Fasta
eg201113_01.msf Msf
eg201113_01.msfView Msf
eg201113_01.pdResult Other
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Publications

Fibrillin-1 regulates endothelial sprouting during angiogenesis.

Alonso Florian F   Dong Yuechao Y   Li Ling L   Jahjah Tiya T   Dupuy Jean-William JW   Fremaux Isabelle I   Reinhardt Dieter P DP   Génot Elisabeth E  

Proceedings of the National Academy of Sciences of the United States of America 20230530 23


Fibrillin-1 is an extracellular matrix protein that assembles into microfibrils which provide critical functions in large blood vessels and other tissues. Mutations in the fibrillin-1 gene are associated with cardiovascular, ocular, and skeletal abnormalities in Marfan syndrome. Here, we reveal that fibrillin-1 is critical for angiogenesis which is compromised by a typical Marfan mutation. In the mouse retina vascularization model, fibrillin-1 is present in the extracellular matrix at the angiog  ...[more]

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