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Determination of MHV RNA poly(A) tail length and 3' end additions in infected 17-CL1 and NCTC cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus Murine hepatitis virus

PROVIDER: GSE200416 | GEO | 2023/03/23

REPOSITORIES: GEO

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Determination of MHV RNA poly(A) tail length and 3' end additions upon 17-CL1 cells infection [17-CL1_WT]

Project description:The mouse hepatitis virus (MHV) genomic and sub-genomic RNAs are 3’ polyadenylated. The length of the 3’ poly(A) tail is known to change during infection, but little is known about the molecular mechanisms driving this change. Here, we investigate the presence of terminal uridylation and terminal guanylation on the MHV poly(A) tail during infection. We infected 17-CL1 cells with MHV and isolated RNA at 24- and 48-hour post-infection (hpi). We observed that MHV RNAs poly(A) tails shortened during infection and that short poly(A) tails of about 20 nucleotides are highly uridylated.

2023-03-23 | GSE200412 | GEO

Determination of MHV RNA poly(A) tail length and 3' end additions upon NCTC cells infection

Project description:The mouse hepatitis virus (MHV) genomic and sub-genomic RNAs have 3’ poly(A) tails. The terminal addition of uridines to poly(A) tails has been shown to be a widespread modification. Here, we investigated the presence of 3' end additions on the MHV RNA poly(A) tails. To this end, we infected NCTC cells with MHV and isolated RNA at 24-hours post-infection (hpi). While the median poly(A) tail length of the MHV RNAs is around 50 nucleotides, we observed a peak of uridylation in transcripts with poly(A) tails about 40 nucleotides long

2023-03-23 | GSE200414 | GEO

Determination of MHV virion genome poly(A) tail length and 3' end additions.

Project description:The mouse hepatitis virus (MHV) genomic RNA has a poly(A) tail required for replication. Here we investigated the presence of terminal poly(A) tail uridylation and guanylation in the virion RNA. After isolating the viral RNA followed by sequencing, we found a mean poly(A) tail of 66 nucleotides long with a peak of terminal uridylation on tails 55 to 66 nucleotides long.

2023-03-23 | GSE200415 | GEO

Determination of MHV RNA poly(A) tail length and 3' end additions in infected 17-CL1 and NCTC cells

Project description:Determination of MHV RNA poly(A) tail length and 3' end additions in infected 17-CL1 and NCTC cells

| PRJNA824468 | ENA

Role of TUT4 and TUT7 on the regulation of MHV poly(A) tail length and 3' end additions [17-CL1_Mutants]

Project description:Terminal uridylation of mRNAs poly(A) tails by Terminal Uridylyl Transferases (TUTs) promote transcript decay. Here, we investigate the role of two functionally redundant mammalian TUTs, TUT4 and TUT7 (TUT4/7), in mouse hepatitis virus (MHV) RNA processing. We generated a TUT4/7 knock-down 17-CL1 cell line using lentivirus encoding shRNAs against Tut4 and Tut7 transcripts (shTUT4/7) and a control 17-CL1 cell line (shCTL) with a non-targeting shRNA. We then infected the shCTL and shTUT4/7 cells with MHV and isolated RNA at 24- and 48-hours post-infection (hpi). We found that viral RNA poly(A) tails of about 20 and 40 nucleotides are highly uridylated and that the uridylation of the 20 nucleotide long tails is dependent on TUT4/7. Depletion of TUT4/7 also resulted in an accumulation of the viral RNA.

2023-03-23 | GSE200413 | GEO

Determination of MHV RNA poly(A) tail length and 3' end additions upon 17-CL1 cells infection [17-CL1_WT]

Project description:Determination of MHV RNA poly(A) tail length and 3' end additions upon 17-CL1 cells infection [17-CL1_WT]

| PRJNA824473 | ENA

Determination of MHV virion genome poly(A) tail length and 3' end additions.

Project description:Determination of MHV virion genome poly(A) tail length and 3' end additions.

| PRJNA824470 | ENA

Determination of MHV RNA poly(A) tail length and 3' end additions upon NCTC cells infection

Project description:Determination of MHV RNA poly(A) tail length and 3' end additions upon NCTC cells infection

| PRJNA824471 | ENA

Role of TUT4 and TUT7 on the regulation of MHV poly(A) tail length and 3' end additions [17-CL1_Mutants]

Project description:Role of TUT4 and TUT7 on the regulation of MHV poly(A) tail length and 3' end additions [17-CL1_Mutants]

| PRJNA824472 | ENA

Nucleophosmin deposition during mRNA 3' end processing influences poly(A) tail length.

Project description:During polyadenylation, the multi-functional protein nucleophosmin (NPM1) is deposited onto all cellular mRNAs analysed to date. Premature termination of poly(A) tail synthesis in the presence of cordycepin abrogates deposition of the protein onto the mRNA, indicating natural termination of poly(A) addition is required for NPM1 binding. NPM1 appears to be a bona fide member of the complex involved in 3' end processing as it is associated with the AAUAAA-binding CPSF factor and can be co-immunoprecipitated with other polyadenylation factors. Furthermore, reduction in the levels of NPM1 results in hyperadenylation of mRNAs, consistent with alterations in poly(A) tail chain termination. Finally, knockdown of NPM1 results in retention of poly(A)(+) RNAs in the cell nucleus, indicating that NPM1 influences mRNA export. Collectively, these data suggest that NPM1 has an important role in poly(A) tail length determination and may help network 3' end processing with other aspects of nuclear mRNA maturation.

| S-EPMC3209774 | biostudies-literature

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