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Egr2 promoter antisense RNA as coordinator of chromatin remodeling and genome reorganization in Schwann cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE201627 | GEO | 2023/04/14

REPOSITORIES: GEO

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Egr2 promoter antisense RNA as coordinator of chromatin remodeling and genome reorganization in Schwann cells [Hi-C]

Project description:The Egr2 promoter antisense RNA (Egr2-AS-RNA) recruits chromatin remodeling complexes to inhibit Egr2 transcription following peripheral nerve injury. Here we show that the Egr2-AS-RNA modulates chromatin accessibility and serves as scaffold for two distinct histone modification complexes. The Egr2-AS-RNA binds to Ezh2 and Wdr5 and enables coordinate targeting of H3K27me3 and H3K4me3 to promoters of Egr2 and c-Jun. Expression of the AS-RNA results in reorganization of the global chromatin landscape and quantitative changes in loop formation and in contact frequency at domain boundaries exhibiting enrichment for AP-1 binding events. Our results show for the first time that the Egr2 promoter AS-RNA may serve as a regulator of coordinate chromatin remodeling and genome organization to regulate transcription, with a significant role in phenotypic plasticity of SCs.

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Egr2 promoter antisense RNA as coordinator of chromatin remodeling and genome reorganization in Schwann cells [ATAC-seq]

2023-04-14 | GSE201624 | GEO

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Egr2 promoter antisense RNA as coordinator of chromatin remodeling and genome reorganization in Schwann cells

Project description:Egr2 promoter antisense RNA as coordinator of chromatin remodeling and genome reorganization in Schwann cells

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Egr2 promoter antisense RNA as coordinator of chromatin remodeling and genome reorganization in Schwann cells [RNA-seq]

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Egr2 promoter antisense RNA as coordinator of chromatin remodeling and genome reorganization in Schwann cells [Hi-C]

Project description:Egr2 promoter antisense RNA as coordinator of chromatin remodeling and genome reorganization in Schwann cells [Hi-C]

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Egr2 promoter antisense RNA as coordinator of chromatin remodeling and genome reorganization in Schwann cells [ATAC-seq]

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Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes.

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