Similar Datasets
Project description:Limitations of a submerged culture system
| PRJNA834644 | ENA
Project description:in vitro microarray study of transcriptional changes of jejunal cells
2018-11-14 | GSE111185 | GEO
Project description:in vitro microarray study of transcriptional changes of jejunal cells
2018-11-14 | GSE111184 | GEO
Project description:in vivo microarray study of transcriptional changes of jejunal scratchings (mucosa) obtained from pigs divergent in feed efficiency.
2018-12-21 | GSE109332 | GEO
Project description:The filamentous fungus Aspergillus terreus is known to produce both industrially and pharmaceutically important secondary metabolites. The objective of this study is to investigate the effect of exogenously added butyrolactone I (BI) on the submerged culture of A. terreus, especially on the possible regulation of the secondary metabolism on the transcriptional level. In order to elucidate the presumed regulative role of butyrolactone I, a large-scale microarray gene expression study was designed and conducted with an industrially utilised A. terreus strain MUCL38669. A. terreus MUCL38669 was cultured in secondary metabolism inducing submerged conditions for nine days, where butyrolactone I was added at the beginning of the growth phase (at 24 hours p.i.), in the middle of the growth phase (at 96 hours p.i.) or in the late growth phase (at 120 hours p.i.), in addition to the control culture where no exogenous butyrolactone I was added. To obtain comprehensive gene expression profiles over the whole culture time, samples were taken at six time points: 24 hours, 48 hours, 96 hours, 120 hours, 144 hours and 216 hours post inoculation.
2017-05-08 | GSE93552 | GEO
Project description:1. The lipids of the red mycelium of the fungus Epicoccum nigrum Link were investigated. Four carotenoid pigments were isolated and identified as beta-carotene, gamma-carotene, rhodoxanthin and torularhodin. 2. For the first time rhodoxanthin was isolated as a fungal metabolite. 3. Linoleic acid was the major fatty acid in the total lipids. 4. Ergosterol was present in the lipids in the non-esterified form.
| S-EPMC1198473 | biostudies-other
Project description:Filamentous fungi are confronted with changes and limitations of their carbon source during growth in their natural habitats and during industrial applications. To survive life-threatening starvation conditions, carbon from extra- and intracellular resources becomes mobilized to fuel fungal self-propagation. Key to understand the underlying cellular processes is the system-wide analysis of fungal starvation responses in a temporal and spatial resolution. The knowledge deduced is important for the development of optimized industrial production processes. This study describes the physiological, morphological and genome-wide transcriptional changes caused by prolonged carbon starvation during submerged batch cultivation of the filamentous fungus Aspergillus niger. Bioreactor cultivation supported highly reproducible growth conditions and monitoring of physiological parameters. Changes in hyphal growth and morphology were analyzed at distinct cultivation phases using automated image analysis. The Affymetrix GeneChip platform was used to establish genome-wide transcriptional profiles for three selected time points during prolonged carbon starvation. Compared to the exponential growth transcriptome, about 50% (7,292) of all genes displayed differential genes expression during at least one of the starvation time points. Enrichment analysis of Gene Ontology, Pfam domain and KEGG pathway annotations uncovered autophagy and asexual reproduction as major global transcriptional trends. Induced transcription of genes encoding hydrolytic enzymes was accompanied by increased secretion of hydrolases including chitinases, glucanases, proteases and phospholipases as identified by mass spectrometry. This study is the first system-wide analysis of the carbon starvation response in a filamentous fungus. Morphological, transcriptomic and secretomic analyses identified key events important for fungal survival and their chronology. The dataset obtained forms a comprehensive framework for further elucidation of the interrelation and interplay of the individual cellular events involved. for each post-exponential time point (Day1, Day3 and Day6 post-carbon depletion), biological duplicates were performed.
2012-08-12 | E-GEOD-39559 | biostudies-arrayexpress
Project description:in vitro microarray study of transcriptional changes of primary monocyte-derived cells
2023-12-18 | GSE250031 | GEO
Project description:Filamentous fungi are confronted with changes and limitations of their carbon source during growth in their natural habitats and during industrial applications. To survive life-threatening starvation conditions, carbon from extra- and intracellular resources becomes mobilized to fuel fungal self-propagation. Key to understand the underlying cellular processes is the system-wide analysis of fungal starvation responses in a temporal and spatial resolution. The knowledge deduced is important for the development of optimized industrial production processes. This study describes the physiological, morphological and genome-wide transcriptional changes caused by prolonged carbon starvation during submerged batch cultivation of the filamentous fungus Aspergillus niger. Bioreactor cultivation supported highly reproducible growth conditions and monitoring of physiological parameters. Changes in hyphal growth and morphology were analyzed at distinct cultivation phases using automated image analysis. The Affymetrix GeneChip platform was used to establish genome-wide transcriptional profiles for three selected time points during prolonged carbon starvation. Compared to the exponential growth transcriptome, about 50% (7,292) of all genes displayed differential genes expression during at least one of the starvation time points. Enrichment analysis of Gene Ontology, Pfam domain and KEGG pathway annotations uncovered autophagy and asexual reproduction as major global transcriptional trends. Induced transcription of genes encoding hydrolytic enzymes was accompanied by increased secretion of hydrolases including chitinases, glucanases, proteases and phospholipases as identified by mass spectrometry. This study is the first system-wide analysis of the carbon starvation response in a filamentous fungus. Morphological, transcriptomic and secretomic analyses identified key events important for fungal survival and their chronology. The dataset obtained forms a comprehensive framework for further elucidation of the interrelation and interplay of the individual cellular events involved.
2012-08-13 | GSE39559 | GEO
Project description:Obesity is associated with an increased risk of mucosal infections; however, the mechanistic basis of this phenomenon remains incompletely defined. Intestinal mucus barrier systems normally prevent infections, but are sensitive to changes in the luminal environment. Here we demonstrate that mice exposed to an obesogenic Western-style diet (WSD) suffer regiospecific failure of the mucus barrier in the small intestinal jejunum caused by diet-induced mucus condensation, which occurs independently of microbiota alterations. Mucus barrier disruption due to either WSD exposure or chromosomal Muc2 deletion results in collapse of the commensal jejunal microbiota, which in turn sensitises mice to atypical jejunal colonization by the enteric pathogen Citrobacter rodentium. We identify the jejunal mucus layer as a microbial habitat, and link the regiospecific mucus dependency of the microbiota to fundamental properties of the jejunal niche. Together, our data identifies a symbiotic mucus-microbiota relationship that normally prevents jejunal pathogen colonization, but is highly sensitive to disruption by exposure to a Western-style diet.
2023-05-12 | PXD028613 | Pride