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Genome-wide gain-of-function screening characterized lncRNAs as tumor immune response regulators

ABSTRACT: RNA-seq data for candidate genes identified from CRISPR activation screen for tumor response regulators

ORGANISM(S): Homo sapiens

PROVIDER: GSE202093 | GEO | 2022/12/12

REPOSITORIES: GEO

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Genome-wide gain-of-function screening characterized lncRNAs as tumor immune response regulators [CRISPR_screen]

Project description:The majority of lncRNAs’ roles in tumor immunology remain elusive. In this project, we performed a CRISPR activation screening of 9744 lncRNAs in melanoma cells co-culturing with human CD8+ T cells. We identified 16 novel lncRNAs potentially regulating tumor immune response. Further integrative analysis using tumor immunogenomics data revealed that IL10RB-DT and LINC01198 are significantly correlated with tumor immune response and survival in melanoma and breast cancer. Specifically, IL10RB-DT suppresses CD8+ T cells activation via inhibiting IFN-γ-JAK-STAT1 signaling and antigen presentation in melanoma and breast cancer cells. On the other hand, LINC01198’s upregulation sensitizes the killing of tumor cells by CD8+ T cells. Mechanistically, LINC01198 interacts and activates NF-κB component p65 to trigger the type I and type II interferon responses in melanoma and breast cancer cells. Our study systematically characterized novel lncRNAs involved in tumor immune response.

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Genome-wide gain-of-function screening characterized lncRNAs as tumor immune response regulators

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Genome-wide gain-of-function screening characterized lncRNAs as tumor immune response regulators [CRISPR_screen]

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