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Real-time quantitative PCR analysis of mouse liver tissue and liver cells

ABSTRACT: Diets rich in fat is a major cause of common chronic liver diseases in worldwide and nutritional food has been widely used to counteract the metabolic disorders such as non-alcoholic fatty liver disease (NAFLD). The present study investigated the effects of oleuropein-enriched extract from Jasminum grandiflorum L. flowers (OLE-JGF), a medicinal plant that is also widely consumed as a beverage in China, in high fat diet (HFD) fed mice and oleic acid (OA)-treated AML-12 cells. Treatment of HFD-fed mice with 0.6% (w/w) OLE-JGF for 8 weeks significantly reduced the plasma levels of ALT, TC and LDL-C without changing the liver weight. Additionally, OLE-JGF administration significantly suppressed the mRNA expressions of the inflammatory mediators MCP-1 and CD68 in the liver of HFD-fed mice. Importantly, treatment with OLE-JGF significantly downregulated the key lipogenic enzymes (ACC and FAS) and their upstream transcription factor SREBP-1c in the liver. In the same time, mitochondrial DNA and UCP2 were upregulated along with increased expression of mitochondrial biogenic promoters including LKB1 and its downstream gene PGC-1α, Nrf2 and Tfam, but not changed AMPK in liver. In vitro, treatment of OLE for 24 h significantly increased the cell viability and decreased the TG level in OA-induced AML-12 cells. OLE significantly inhibited ACC mRNA expression and upregulated LKB1, PGC-1α and Tfam mRNA levels in OA-treated cells. In addition, OLE significantly increased the binding level of LKB1 to PGC-1α promoter in OA-induced cells. These findings indicate that OLE-JGF attenuates hepatocyte damage in HFD-fed mice and OA-induced liver cells, may be partly attributed to upregulation of LKB1-PGC-1α axis, which mediates hepatic lipogenesis and mitochondrial biogenesis. Our study provides a scientific basis for the benefits of J. grandiflorum flower as a supplement for met-abolic disease and potential use of OLE for the treatment of chronic liver diseases.

ORGANISM(S): Mus musculus Mus

PROVIDER: GSE202707 | GEO | 2022/05/14

REPOSITORIES: GEO

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Project description:Title: Total Skeletal Muscle PGC-1 Deficiency Uncouples Mitochondrial Derangements from Fiber Type Determination and Insulin Sensitivity Abstract: Evidence is emerging that the PGC-1 coactivators serve a critical role in skeletal muscle metabolism, function, and disease. Mice with total PGC-1 deficiency in skeletal muscle (PGC-1α-/- βf/f/MLC-Cre mice) were generated and characterized. PGC-1α-/-βf/f/MLC-Cre mice exhibit a dramatic reduction in exercise performance compared to single PGC-1α- or PGC-1β-deficient mice and wild-type controls. The exercise phenotype of the PGC-1α-/-βf/f/MLC-Cre mice was associated with a marked diminution in muscle oxidative capacity and mitochondrial structural derangements consistent with fusion/fission and biogenic defects together with rapid depletion of muscle glycogen stores during exercise. Surprisingly, the skeletal muscle fiber type profile of the PGC-1α-/-βf/f/MLCCre mice was not significantly different than the wild-type mice. Moreover, insulin sensitivity and glucose tolerance were also not altered in the PGC-1α-/-βf/f/MLC-Cre mice. Taken together, we conclude that PGC-1 coactivators are necessary for the oxidative and mitochondrial programs of skeletal muscle but are dispensable for fundamental fiber type determination and insulin sensitivity. RNA from PGC-1alpha-/- beta f/f/Mlc1fcre was obtained and gene expression pattern compared with PGC-1alpha -/-, PGC-1beta f/f, and PGC-1beta f/f/Mlc1fCre controls. Results file descriptions: 1. GSE23365_skfloxAKO_PPexcl_genesup_GEO-8-16-2010: This table contains genes that were upregulated ≥2.0 fold in gastrocnemius muscle from PGC-1alpha-/- - mice, PGC-1beta f/f/Mlc1fCre mice and PGC-1alpha-/- - beta f/f/Mlc1fCre mice. All groups are normalized to PGC-1beta f/f mice and values are expressed as mean±SEM. The column “description’ contains the gene name, and the column “ID” contains Agilent probe names. 2. GSE23365_skfloxAKO_PPexcl_genesdown_GEO-8-16-2010 This table contains genes that were downregulated ≤0.7 fold in gastrocnemius muscle from PGC-1alpha-/- - mice, PGC-1beta f/f/Mlc1fCre mice and PGC-1alpha-/- - beta f/f/Mlc1fCre mice. All groups are normalized to PGC-1beta f/f mice and values are expressed as mean±SEM. The column “description’ contains the gene name, and the column “ID” contains Agilent probe names.

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Real-time quantitative PCR analysis of mouse liver tissue and liver cells

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