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Igh repertoire analysis in primary pro-B cells from WT and NE1-/- mice (VDJ-seq)

ABSTRACT: Analysis of the antibody repertoire composition is now possible using VDJ-seq. We used this recently developed method for unbiased amplification from genomic DNA (gDNA) to directly compare the Igh repertoire of C57Bl/6 (WT) and NE1-/- pro-B cells. We find that a group of contiguous proximal and intermediate VH genes are under-utilized in V->DJ rearrangement in the absence of NE1 revealing a NE1 zone of influence. We report the VH gene usage profile from WT and NE1-/- primary pro-B cells from the VDJ-seq data.

ORGANISM(S): Mus musculus Mus

PROVIDER: GSE203484 | GEO | 2022/12/22

REPOSITORIES: GEO

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Igh repertoire analysis in primary pro-B cells from WT and NE1-/- mice (VDJ-seq)

Project description:Igh repertoire analysis in primary pro-B cells from WT and NE1-/- mice (VDJ-seq)

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