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A single-round of antigen receptor signaling programs naïve B cells to receive T cell help

ABSTRACT: To simulate transient B cell activation that is the likely initiator of T-dependent responses, we examined the molecular and functional consequences of a single-round of immunoglobulin M (IgM) signaling. This form of activation triggered early cytosolic signaling and transcription factor NF-kB activation indistinguishably from conventional continuous IgM cross-linking, but did not induce G1 progression. However, single-round IgM signaling changed the expression of chemokine and chemokine receptor genes implicated in initiating T-dependent responses, as well as accentuated responsiveness to CD40 signaling. Several features of single-round IgM signaling in vitro were recapitulated in B cells after short-term exposure to antigen in vivo. We propose that transient BCR signals prime B cells to receive T cell help by increasing the probability of B-T encounter and creating a cellular environment that is hyper-responsive to CD40 signaling.

ORGANISM(S): Mus musculus

PROVIDER: GSE20477 | GEO | 2010/05/24

SECONDARY ACCESSION(S): PRJNA125161

REPOSITORIES: GEO

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A single-round of antigen receptor signaling programs naÃ¯ve B cells to receive T cell help

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