Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Methods for Culturing Adult CNS Neurons Reveal a CNS Conditioning Effect

ABSTRACT: To develop a successful, reliable and scalable method for establishing primary cultures of adult neurons from all regions of the brain. Using adult motor cortex cultures, we identify a CNS "conditioning" effect after spinal cord injury. In vivo conditioning was administered 24hrs prior to cultures.

ORGANISM(S): Mus musculus

PROVIDER: GSE205486 | GEO | 2022/06/06

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Methods for Culturing Adult CNS Neurons Reveal a CNS Conditioning Effect

Project description:Methods for Culturing Adult CNS Neurons Reveal a CNS Conditioning Effect

| PRJNA845938 | ENA

Methods for culturing adult CNS neurons reveal a CNS conditioning effect.

Project description:Neuronal cultures provide a basis for reductionist insights that rely on molecular and pharmacological manipulation. However, the inability to culture mature adult CNS neurons limits our understanding of adult neuronal physiology. Here, we report methods for culturing adult central nervous system neurons in large numbers and across multiple brain regions for extended time periods. Primary adult neuronal cultures develop polarity; they establish segregated dendritic and axonal compartments, maintain resting membrane potentials, exhibit spontaneous and evoked electrical activity, and form neural networks. Cultured adult neurons isolated from different brain regions such as the hippocampus, cortex, brainstem, and cerebellum exhibit distinct cell morphologies, growth patterns, and spontaneous firing characteristics reflective of their regions of origin. Using adult motor cortex cultures, we identify a CNS "conditioning" effect after spinal cord injury. The ability to culture adult neurons offers a valuable tool for studying basic and therapeutic science of the brain.

| S-EPMC9308166 | biostudies-literature

Conditioning-1

Project description:Conditioning-1

| PRJNA1074489 | ENA

Npas4 CUT&Tag in amygdala tissue after discriminative fear conditioning

Project description:Npas4 CUT&Tag dataset, Adult male WT C57BL/6J mice underwent discriminative fear conditioning and immediately injected saline. 90 minutes after fear conditioning, The amygdala tissue was extracted for further experiment. Npas4 CUT&Tag was performed to elucidate possible downstream targets which contributes regulation of fear expression during fear retreival.

2023-07-05 | GSE194069 | GEO

Bottle conditioning - yeast strain

Project description:LC-MS of yeast strain that maintained high viability during bottle conditioning compared to strain that had poor viability during bottle conditioning.

2024-10-17 | PXD053930 | Pride

Dorsal hippocampal transcriptome analysis after Auditory Fear Conditioning and pharmacological GR modulation

Project description:Aim: We investigated the role of GR in Auditory Fear Conditioning memory consolidation by application of a pharmacological filter using selective glucocorticoid receptor modulators. Methods: Adult male mice were exposed to Auditory Fear Conditioning training and subsequently injected with 3.0 mg/kg corticosterone, 20 mg/kg CORT108297, 80 mg/kg CORT118335, 40 mg/kg RU486 or vehicle. Dorsal hippocampi were dissected 3 hours after injection and snap-frozen. Total RNA was isolated and send for 100bp paired-end sequencing by BGI.

2023-05-01 | GSE202236 | GEO

air-conditioning system dust

Project description:air-conditioning system dust Other

| PRJNA488116 | ENA

RNA-seq analysis of contextual fear conditioning

Project description:This dataset constitutes the first RNA-seq study of gene expression following contextual fear conditioning in the mouse hippocampus.

2015-07-22 | GSE63412 | GEO

Sono-Seq analysis of contextual fear conditioning

Project description:This dataset constitutes the first Sono-Seq study of chromatin accessibility following contextual fear conditioning in the mouse hippocampus.

2017-11-20 | GSE98317 | GEO

Ultrasonic surgical aspirate is a reliable source for culturing glioblastoma stem cells

Project description:Glioblastoma stem cells (GSCs) are thought to be the source of tumor growth and therapy resistance. Core biosies that are used to generate GSC cultures are ususally taken from one part of the tumor and are thus unlikely to represent intra-tumoral heterogeneity. This study shows that the ultrasonic aspirates (UA), that are usually considered as a biological waste, can be used as a reliable source of GSCs. Furthermore the UA aspirates seem to be capturing the tumorigenic signature better than the traditional biopsies.

2017-02-10 | GSE84707 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data