Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Methods for Culturing Adult CNS Neurons Reveal a CNS Conditioning Effect

ABSTRACT: Methods for Culturing Adult CNS Neurons Reveal a CNS Conditioning Effect

PROVIDER: PRJNA845938 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19542900_1.fastq.gz	Fastqsanger.gz
	SRR19542900_2.fastq.gz	Fastqsanger.gz
	SRR19542901_1.fastq.gz	Fastqsanger.gz
	SRR19542901_2.fastq.gz	Fastqsanger.gz
	SRR19542902_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 58

Similar Datasets

Methods for Culturing Adult CNS Neurons Reveal a CNS Conditioning Effect

Project description:To develop a successful, reliable and scalable method for establishing primary cultures of adult neurons from all regions of the brain. Using adult motor cortex cultures, we identify a CNS "conditioning" effect after spinal cord injury. In vivo conditioning was administered 24hrs prior to cultures.

2022-06-06 | GSE205486 | GEO

Methods for culturing adult CNS neurons reveal a CNS conditioning effect.

Project description:Neuronal cultures provide a basis for reductionist insights that rely on molecular and pharmacological manipulation. However, the inability to culture mature adult CNS neurons limits our understanding of adult neuronal physiology. Here, we report methods for culturing adult central nervous system neurons in large numbers and across multiple brain regions for extended time periods. Primary adult neuronal cultures develop polarity; they establish segregated dendritic and axonal compartments, maintain resting membrane potentials, exhibit spontaneous and evoked electrical activity, and form neural networks. Cultured adult neurons isolated from different brain regions such as the hippocampus, cortex, brainstem, and cerebellum exhibit distinct cell morphologies, growth patterns, and spontaneous firing characteristics reflective of their regions of origin. Using adult motor cortex cultures, we identify a CNS "conditioning" effect after spinal cord injury. The ability to culture adult neurons offers a valuable tool for studying basic and therapeutic science of the brain.

| S-EPMC9308166 | biostudies-literature

Conditioning-1

Project description:Conditioning-1

| PRJNA1074489 | ENA

Bottle conditioning - yeast strain

Project description:LC-MS of yeast strain that maintained high viability during bottle conditioning compared to strain that had poor viability during bottle conditioning.

2024-10-17 | PXD053930 | Pride

Contextual fear conditioning induced transcriptional changes in hippocampal CA1 neurons

Project description:The goal of our study was to assess whether the experience can regulate specific lncRNAs within the hippocampus and their role in associative memory. To address this, we carried out unbiased analyses of gene expression in CA1-hippocampal neurons to identify lncRNA changes induced by contextual fear conditioning (CFC).

2023-08-07 | GSE214838 | GEO

Dorsal hippocampal transcriptome analysis after Auditory Fear Conditioning and pharmacological GR modulation

Project description:Aim: We investigated the role of GR in Auditory Fear Conditioning memory consolidation by application of a pharmacological filter using selective glucocorticoid receptor modulators. Methods: Adult male mice were exposed to Auditory Fear Conditioning training and subsequently injected with 3.0 mg/kg corticosterone, 20 mg/kg CORT108297, 80 mg/kg CORT118335, 40 mg/kg RU486 or vehicle. Dorsal hippocampi were dissected 3 hours after injection and snap-frozen. Total RNA was isolated and send for 100bp paired-end sequencing by BGI.

2023-05-01 | GSE202236 | GEO

air-conditioning system dust

Project description:air-conditioning system dust Other

| PRJNA488116 | ENA

Distinct proteomic brain states underlying long-term memory formation in aversive operant conditioning

Project description:This dataset contains 48 raw files corresponding to repeat runs of 12 peptide digests of whole Lymnaea stagnalis CNS from LTM (denoted as "L"), no LTM (denoted as "NL"), and yoke control (denoted as "Y") animals collected 24 hours following aversive operant conditioning (n=4 animals per group). Database files contain the predicted Lymnaea stagnalis proteome used for searches as well as the reference mouse proteome used for annotation of Lymnaea stagnalis sequences.

2024-04-08 | MSV000094487 | MassIVE

Contextual fear conditioning induced transcriptional changes in hippocampal CA1 neurons

Project description:Contextual fear conditioning induced transcriptional changes in hippocampal CA1 neurons

| PRJNA887379 | ENA

Effect of LARK overexpression in CNS neurons

Project description:The goal of this study is to identify, in the head of adult flies, mRNA species whose expresson level are altered by overexpression of the Drosophila RNA-binding protein LARK in CNS neurons. Keywords: genetic modification, gene experssion profile

2006-12-30 | GSE6420 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data