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Transcriptional Profiling of Host Gene Expression in Chicken Embryo Lung Cells Infected with Laryngotracheitis Virus

ABSTRACT: Transcriptional profiling of chicken embryo lung cells infected infectious laryngotracheitis virus (ILTV) comparing control uninfected lung cells. Goal was to determine the changes of host gene expression by ILTV infection and host-virus interaction.

ORGANISM(S): Gallus gallus

PROVIDER: GSE20630 | GEO | 2010/03/11

SECONDARY ACCESSION(S): PRJNA124845

REPOSITORIES: GEO

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Transcriptional Profiling of Host Gene Expression in Chicken Embryo Lung Cells Infected with Laryngotracheitis Virus

Project description:Transcriptional profiling of chicken embryo lung cells infected infectious laryngotracheitis virus (ILTV) comparing control uninfected lung cells. Goal was to determine the changes of host gene expression by ILTV infection and host-virus interaction. Two-condition experiment, uninfected chicken embryo lung cells vs. infected chicken embryo lung cells. Biological replicates: 1 control uninfected sample, 1 infected experimental sample at each 1dpi, 3dpi, 5dpi, and 7 dpi.

2010-03-10 | E-GEOD-20630 | biostudies-arrayexpress

An integrated transcriptome analysis to the characterization of host responses against infectious laryngostracheitis virus vaccine infection in chicken embryo lung cells

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An integrated transcriptome analysis to the characterization of host responses against infectious laryngostracheitis virus vaccine infection in chicken embryo lung cells

Project description:Host gene expression of chicken embryo lung cells infected an infectious laryngotracheitis virus (ILTV) vaccine strain comparing control uninfected lung cells. Goal was to identify the changes of host gene expression by ILTV vaccine infection. Two-condition experiment, uninfected chicken embryo lung cells vs. vaccine infected chicken embryo lung cells. Biological replicates: 1 control uninfected sample, 1 vaccinal ILTV infected experimental sample at each 1, 2, 3 and 4 dpi.

2011-06-29 | E-GEOD-30269 | biostudies-arrayexpress

Development of gene expression signatures for chicken LMH cells infected with infectious laryngotracheitis virus (ILTV)

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Development of gene expression signatures for chicken LMH cells infected with infectious laryngotracheitis virus (ILTV)

Project description:To explore the molecular events in LMH cells upon infectious laryngotracheitis virus (ILTV) infection, transcriptional profiling of LMH cells infected with ILTV was compared with uninfected control cells. Gene expression in LMH cells with or without virus infection was measured at two day post infection. Four independent experiments were performed.

2015-10-24 | E-GEOD-70855 | biostudies-arrayexpress

Transcriptome analysis reveals an activation of MHC-I and MHC-II pathways in chicken trachea immunized with infectious laryngotracheitis virus vaccine

Project description:Infectious laryngotracheitis (ILT) is an acute, contagious, upper respiratory disease, which is caused by gallid herpesvirus 1 (GaHV-1). Due to the mortality rates up to 70% depending on the virulence of the virus, it is of economic importance of the disease to explore the etiology of the ILT in the poultry industry. In this study, 15-day-old SPF white leghorn chickens were used to transcriptome analysis in chicken trachea immunized with infectious laryngotracheitis virus vaccine. In conclusion, chicken embryo origin (CEO) vaccine activation of the MHC-I and MHC-II pathways provides insight into the molecular mechanism of immune response in chickens, and holds potential for evaluation and design of new ILT vaccines in a manner adapted to the host immune response to the virus.

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Transcriptome analysis reveals an activation of MHC-I and MHC-II pathways in chicken trachea immunized with infectious laryngotracheitis virus vaccine

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Project description:Development of gene expression signatures for chicken LMH cells infected with infectious laryngotracheitis virus (ILTV)

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Infectious laryngotracheitis virus induces paracrine apoptosis in uninfected cells through repression of p53 signalling

Project description:We sequenced mRNA from LMH cells infected with ILTV, uninfected apoptotic cells and apoptotic cells cultured in conditioned medium.

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Transcription profiling time series of chicken cell culturs infected with Chicken Anaemia Virus CAV

Project description:Chicken anaemia virus (CAV) causes severe anaemia and immunosuppression in young chickens. Such effects reduce the efficiency of routine vaccinations while aggravating the effects of other pathogens in chicken populations. So far, the host responses to CAV have not been studied in vivo or in vitro on a whole genome-wide scale. In this study, we compared gene expression profiles of uninfected and CAV infected cell cultures (MDCC-MSB1 cells-in vitro) at 24, 48 and 72 hr post infection. A chicken specific-immune microarray (5k) developed at Roslin Institute was used throughout the study. Statistically significant gene expression changes occurred mainly after 72 hr (in vitro).

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