Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis reveals an activation of MHC-I and MHC-II pathways in chicken trachea immunized with infectious laryngotracheitis virus vaccine

ABSTRACT: Infectious laryngotracheitis (ILT) is an acute, contagious, upper respiratory disease, which is caused by gallid herpesvirus 1 (GaHV-1). Due to the mortality rates up to 70% depending on the virulence of the virus, it is of economic importance of the disease to explore the etiology of the ILT in the poultry industry. In this study, 15-day-old SPF white leghorn chickens were used to transcriptome analysis in chicken trachea immunized with infectious laryngotracheitis virus vaccine. In conclusion, chicken embryo origin (CEO) vaccine activation of the MHC-I and MHC-II pathways provides insight into the molecular mechanism of immune response in chickens, and holds potential for evaluation and design of new ILT vaccines in a manner adapted to the host immune response to the virus.

ORGANISM(S): Gallus gallus

PROVIDER: GSE46046 | GEO | 2014/04/08

SECONDARY ACCESSION(S): PRJNA197130

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptome analysis reveals an activation of MHC-I and MHC-II pathways in chicken trachea immunized with infectious laryngotracheitis virus vaccine

Project description:Infectious laryngotracheitis (ILT) is an acute, contagious, upper respiratory disease, which is caused by gallid herpesvirus 1 (GaHV-1). Due to the mortality rates up to 70% depending on the virulence of the virus, it is of economic importance of the disease to explore the etiology of the ILT in the poultry industry. In this study, 15-day-old SPF white leghorn chickens were used to transcriptome analysis in chicken trachea immunized with infectious laryngotracheitis virus vaccine. In conclusion, chicken embryo origin (CEO) vaccine activation of the MHC-I and MHC-II pathways provides insight into the molecular mechanism of immune response in chickens, and holds potential for evaluation and design of new ILT vaccines in a manner adapted to the host immune response to the virus. Ten vaccine inoculated birds were randomly divided in two groups. Each group represents one replication of five pooled tissues, for inoculated birds. Control group consists of five birds that received sterile vaccine diluent.

2014-04-08 | E-GEOD-46046 | biostudies-arrayexpress

Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine

Project description:In this study we investigated the methylome of chickens immunized with Infectious laryngotracheitis (ILT) vaccine derived from chicken embryos. Methyl-CpG binding domain protein-enriched genome sequencing (MBD-Seq) method was employed in the detection of the 1,155 differentially methylated regions (DMRs) across the entire genome. After validation, we ascertained the genomic DMRs distribution and annotated them regarding genes, transcription start sites (TSS) and CpG islands. We found that global DNA methylation decreased in vaccinated birds, presenting 704 hypomethylated and 451 hypermethylated DMRs, respectively. Additionally, we performed an enrichment analysis detecting gene networks, in which cancer and RNA post-transcriptional modification appeared in the first place, followed by humoral immune response, immunological disease and inflammatory disease. The top four identified canonical pathways were EIF2 signaling, regulation of EIF4 and p70S6K signaling, axonal guidance signaling and mTOR signaling, providing new insight regarding the mechanisms of ILT etiology. Lastly, the association between DNA methylation and differentially expressed genes was examined, and detected negative correlation in seventeen of the eighteen genes. DNA methylation analysis employing MBD-Seq with 3 salt concentrations, in vaccinated and control group of chickens with 2 biological replications

2014-08-28 | E-GEOD-56984 | biostudies-arrayexpress

Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine

2014-08-28 | GSE56984 | GEO

Transcriptional Profiling of Host Gene Expression in Chicken Embryo Lung Cells Infected with Laryngotracheitis Virus

2010-03-11 | GSE20630 | GEO

An integrated transcriptome analysis to the characterization of host responses against infectious laryngostracheitis virus vaccine infection in chicken embryo lung cells

2011-06-29 | GSE30269 | GEO

Transcriptional Profiling of Host Gene Expression in Chicken Embryo Lung Cells Infected with Laryngotracheitis Virus

Project description:Transcriptional profiling of chicken embryo lung cells infected infectious laryngotracheitis virus (ILTV) comparing control uninfected lung cells. Goal was to determine the changes of host gene expression by ILTV infection and host-virus interaction. Two-condition experiment, uninfected chicken embryo lung cells vs. infected chicken embryo lung cells. Biological replicates: 1 control uninfected sample, 1 infected experimental sample at each 1dpi, 3dpi, 5dpi, and 7 dpi.

2010-03-10 | E-GEOD-20630 | biostudies-arrayexpress

An integrated transcriptome analysis to the characterization of host responses against infectious laryngostracheitis virus vaccine infection in chicken embryo lung cells

Project description:Host gene expression of chicken embryo lung cells infected an infectious laryngotracheitis virus (ILTV) vaccine strain comparing control uninfected lung cells. Goal was to identify the changes of host gene expression by ILTV vaccine infection. Two-condition experiment, uninfected chicken embryo lung cells vs. vaccine infected chicken embryo lung cells. Biological replicates: 1 control uninfected sample, 1 vaccinal ILTV infected experimental sample at each 1, 2, 3 and 4 dpi.

2011-06-29 | E-GEOD-30269 | biostudies-arrayexpress

Development of gene expression signatures for chicken LMH cells infected with infectious laryngotracheitis virus (ILTV)

2015-10-24 | GSE70855 | GEO

Development of gene expression signatures for chicken LMH cells infected with infectious laryngotracheitis virus (ILTV)

Project description:To explore the molecular events in LMH cells upon infectious laryngotracheitis virus (ILTV) infection, transcriptional profiling of LMH cells infected with ILTV was compared with uninfected control cells. Gene expression in LMH cells with or without virus infection was measured at two day post infection. Four independent experiments were performed.

2015-10-24 | E-GEOD-70855 | biostudies-arrayexpress

Chicken MHC class II presented Marek's disease virus (MDV) and infectious bursal disease virus (IBDV) antigenic peptides

Project description:Viral diseases pose major threats to humans and other animals, including the billions of chickens that are an important food source as well as a public health concern due to zoonotic pathogens. Unlike humans and other typical mammals, the major histocompatibility complex (MHC) of chickens can confer decisive resistance or susceptibility to many viruses. Examples are Marek's disease virus (MDV) and Infectious bursal disease virus (IBDV). We used a new in vitro infection system and immunopeptidomics to identify peptides presented to T lymphocytes via classical MHC class II molecules.

2021-09-10 | PXD023954 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data