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Matrix from Urine Stem Cells Boosts Tissue-Specific Stem Cell Mediated Functional Cartilage Reconstruction

ABSTRACT: Articular cartilage has a limited capacity to self-heal once damaged. Tissue-specific stem cells are a solution for cartilage regeneration; however, ex vivo expansion resulting in cell senescence remains a challenge as a large quantity of high-quality tissue-specific stem cells are needed for cartilage regeneration. Our previous report demonstrated that decellularized extracellular matrix (dECM) deposited by human synovium-derived stem cells (SDSCs), adipose-derived stem cells (ADSCs), urine-derived stem cells (UDSCs), or dermal fibroblasts (DFs) provided a solution to rejuvenate human SDSCs in proliferation and chondrogenic potential. This study focused on the evaluation of ex vivo rejuvenation of rabbit infrapatellar fat pad-derived stem cells (IPFSCs) by the abovementioned dECMs to be used in functional cartilage repair in a rabbit osteochondral defect model and the potential cellular and molecular mechanisms underlying this rejuvenation. We found that dECM rejuvenation promoted rabbit IPFSCs’ cartilage engineering and functional regeneration in both in vitro and in vivo models, particularly for the dECM deposited by UDSCs, which was further confirmed by proteomics data. RNASeq analysis indicated that both mesenchymal-epithelial transition (MET) and inflammation-mediated macrophage activation and polarization are potentially involved in the dECM-mediated promotion of IPFSCs’ chondrogenic capacity, which needs further investigation.

ORGANISM(S): Oryctolagus cuniculus

PROVIDER: GSE207804 | GEO | 2022/11/29

REPOSITORIES: GEO

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