Dataset Information

Proteome Analysis of stromal cells embedded in nanocomposite hydrogels stimulated with ultrasound

ABSTRACT: The regeneration of cartilage is a crucial aspect in the treatment of osteoarthritis. In recent years, cartilage regeneration has been pursued through several bioengineering strategies and using different stem cell types, such as adipose tissue-derived mesenchymal stromal cells (ASCs), however with a rather inefficient expression of key chondrogenic markers. Here, we analyzed the proteome of ASCs embedded in a nanocomposite hydrogel stimulated with ultrasound waves, focusing on pathways related to cartilage regeneration.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Manuela Piazzi

LAB HEAD: Dr Gina Lisignoli

PROVIDER: PXD038147 | Pride | 2024-05-24

REPOSITORIES: Pride

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			Action	DRS
	20220530_ADSC4_HyperG-1002119_07.raw	Raw
	20220530_ADSC4_HyperG-1002119_17.raw	Raw
	20220530_ADSC4_HyperG-1002119_23.raw	Raw
	20220530_ADSC6_HyperG-1002119_09.raw	Raw
	20220530_ADSC6_HyperG-1002119_20.raw	Raw

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Publications

Ultrasound Stimulation of Piezoelectric Nanocomposite Hydrogels Boosts Chondrogenic Differentiation <i>in Vitro</i>, in Both a Normal and Inflammatory Milieu.

Ricotti Leonardo L Cafarelli Andrea A Manferdini Cristina C Trucco Diego D Vannozzi Lorenzo L Gabusi Elena E Fontana Francesco F Dolzani Paolo P Saleh Yasmin Y Lenzi Enrico E Columbaro Marta M Piazzi Manuela M Bertacchini Jessika J Aliperta Andrea A Cain Markys M Gemmi Mauro M Parlanti Paola P Jost Carsten C Fedutik Yirij Y Nessim Gilbert Daniel GD Telkhozhayeva Madina M Teblum Eti E Dumont Erik E Delbaldo Chiara C Codispoti Giorgia G Martini Lucia L Tschon Matilde M Fini Milena M Lisignoli Gina G

ACS nano 20240102 3

The use of piezoelectric nanomaterials combined with ultrasound stimulation is emerging as a promising approach for wirelessly triggering the regeneration of different tissue types. However, it has never been explored for boosting chondrogenesis. Furthermore, the ultrasound stimulation parameters used are often not adequately controlled. In this study, we show that adipose-tissue-derived mesenchymal stromal cells embedded in a nanocomposite hydrogel containing piezoelectric barium titanate nanop ...[more]

PMID: 38166155

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Project description:We have previously reported that the deficiency of p53 alone or in combination with Rb (Rb-/- p53-/-) in adipose-derived MSCs (ASCs) promotes leiomyosarcoma-like tumors in vivo. Here, we hypothesized that the source of MSCs and/or the cell differentiation stage could determine the phenotype of sarcoma development. To investigate whether there is a link between the source of MSCs and sarcoma phenotype, we generated p53-/- and Rb-/-p53-/- MSCs from bone marrow (BM-MSCs). Both genotypes of BM-MSCs initiated leiomyosarcoma formation similar to p53-/- and Rb-/-p53-/- ASCs. In addition, gene expression profiling revealed a link between p53- or Rb-p53-deficient BM-MSCs and ASCs and muscle-associated sarcomagenesis. These data suggest that the tissue source of MSC does not seem a crucial factor in the development of a particular sarcoma phenotype. To analyze whether the differentiation stage defines the sarcoma phenotype, BM-MSCs and ASCs were induced to differentiate towards the osteogenic lineage, and both p53 and Rb were excised using Cre-expressing adenovectors at different stages along osteogenic differentiation. Regardless of the level of osteogenic commitment, the inactivation of Rb and p53 in BM-MSC-derived, but not in ASC-derived, osteogenic progenitors gave rise to osteosarcoma-like tumors which could be serially transplanted. This indicates that the osteogenic differentiation stage of BM-MSCs imposes the phenotype of in vivo sarcoma development, and that BM-MSC-derived osteogenic progenitors rather than undifferentiated BM-MSCs, undifferentiated ASCs or ASC-derived osteogenic progenitors, represent the cell of origin for osteosarcoma development. To analyse whether the BM-MSC and Fat-MSC (ASC) differentiation stage may define the sarcoma phenotype, RbloxP/loxPp53loxP/loxP BM-MSCs and ASCs were induced to differentiate towards the osteogenic lineage and both Rb and p53 were excised with adenoviral vectors expressing the Cre-recombinase gene (Ad-CMV-Cre) at different stages (day 0 and 10) along osteogenic differentiation. NSG mice were inoculated subcutaneously with 5M-CM-^W10^6 mutant cells. Animals were killed when tumors reached 1 cm3 or 150 days after infusion. Some of the obtained tumors were mechanically disaggregated to establish ex vivo MSC-transformed cell lines. Gene expression analysis was performed using: WT BM-MSCs and ASCs, Rb-/-p53-/- BM-MSCs and ASCs previously differentiated to the osteogenic lineage for 10 days and a tumor cell line derived from p53-/-Rb-/- BM-MSC differentiated to the osteogenic lineage for 10 days.

Project description:We have previously reported that the deficiency of p53 alone or in combination with Rb (Rb-/- p53-/-) in adipose-derived MSCs (ASCs) promotes leiomyosarcoma-like tumors in vivo. Here, we hypothesized that the source of MSCs and/or the cell differentiation stage could determine the phenotype of sarcoma development. To investigate whether there is a link between the source of MSCs and sarcoma phenotype, we generated p53-/- and Rb-/-p53-/- MSCs from bone marrow (BM-MSCs). Both genotypes of BM-MSCs initiated leiomyosarcoma formation similar to p53-/- and Rb-/-p53-/- ASCs. In addition, gene expression profiling revealed a link between p53- or Rb-p53-deficient BM-MSCs and ASCs and muscle-associated sarcomagenesis. These data suggest that the tissue source of MSC does not seem a crucial factor in the development of a particular sarcoma phenotype. To analyze whether the differentiation stage defines the sarcoma phenotype, BM-MSCs and ASCs were induced to differentiate towards the osteogenic lineage, and both p53 and Rb were excised using Cre-expressing adenovectors at different stages along osteogenic differentiation. Regardless of the level of osteogenic commitment, the inactivation of Rb and p53 in BM-MSC-derived, but not in ASC-derived, osteogenic progenitors gave rise to osteosarcoma-like tumors which could be serially transplanted. This indicates that the osteogenic differentiation stage of BM-MSCs imposes the phenotype of in vivo sarcoma development, and that BM-MSC-derived osteogenic progenitors rather than undifferentiated BM-MSCs, undifferentiated ASCs or ASC-derived osteogenic progenitors, represent the cell of origin for osteosarcoma development. BM-MSC and ASC cultures were established from 3 mouse strains: (a) WT, (b) p53loxP/loxP and (c) p53loxP/loxP RbloxP/loxP. The corresponding mutant cells were generated by excision of the LoxP-flanked sequences by infection of all MSC cultures with adenoviral vectors expressing the Cre-recombinase gene (Ad-CMV-Cre). NSG mice were inoculated subcutaneously with 5×10^6 mutant cells. Animals were killed when tumors reached 1 cm3 or 150 days after infusion. Some of the obtained tumors were mechanically disaggregated to establish ex vivo MSC-transformed cell lines. Gene expression analysis was performed using BM-MSCs and ASCs from all genotypes, as well as tumor cell lines derived from p53-/- and p53-/-Rb-/- BM-MSC and ASC cultures.

Dataset Information

Proteome Analysis of stromal cells embedded in nanocomposite hydrogels stimulated with ultrasound

Dataset's files

Publications

Ultrasound Stimulation of Piezoelectric Nanocomposite Hydrogels Boosts Chondrogenic Differentiation <i>in Vitro</i>, in Both a Normal and Inflammatory Milieu.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Proteome Analysis of stromal cells embedded in nanocomposite hydrogels stimulated with ultrasound

Dataset's files

Publications

Ultrasound Stimulation of Piezoelectric Nanocomposite Hydrogels Boosts Chondrogenic Differentiation &lt;i&gt;in Vitro&lt;/i&gt;, in Both a Normal and Inflammatory Milieu.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Ultrasound Stimulation of Piezoelectric Nanocomposite Hydrogels Boosts Chondrogenic Differentiation <i>in Vitro</i>, in Both a Normal and Inflammatory Milieu.