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Effect of bantam knockdown on mRNA levels in the the γ5β′2a/β′2mp/β′2mp_bilateral Mushroom Body Output Neurons (MBONs) in Drosophila Melanogaster

ABSTRACT: In order to knockdown bantam in the γ5β′2a/β′2mp/β′2mp_bilateral Mushroom Body Output Neurons (MBONs), we expressed the bantam microRNA-sponge (or the control scrambled-sponge) under the MB011B-split-Gal4 driver. Additionally, we expressed a membrane localized GFP transgene. At ZT12-13.5, MB011B>ban-spg, GFP and MB011B>scramble-spg, GFP female fly brains were diseccted, homogenized and FACS sorted using GFP flourescence to isolate the MBONs of interest. mRNA was extracted and used to construct Illumina RNAseq libraries

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE208649 | GEO | 2022/07/21

REPOSITORIES: GEO

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