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A nuclear-localized red fluorescence knock-in reporter allele for mouse pancreatic beta-cells

ABSTRACT: In this study, we generated a novel nuclear-localized red fluorescence knock-in reporter allele (Ins2.Apple) for mouse pancreatic beta-cells. Beta-cells were isolated by FACS from 60-day-old mice, segregated by sex, and RNA-sequencing was performed to assess sex-specific differences in beta-cell gene expression profiles. We also isolated beta-cells (by FACS) from MIP-GFP mice at 60 days of age. RNA-sequencing was performed, and was compared to that of Ins2.Apple beta cells, to assess gene expression changes brought on by the presence of the MIP-GFP transgene.

INSTRUMENT(S): Illumina HiSeq 3000

ORGANISM(S): Mus musculus

SUBMITTER: Jennifer Stancill

PROVIDER: E-MTAB-6329 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transgene-associated human growth hormone expression in pancreatic β-cells impairs identification of sex-based gene expression differences.

Stancill Jennifer S JS Osipovich Anna B AB Cartailler Jean-Philippe JP Magnuson Mark A MA

American journal of physiology. Endocrinology and metabolism 20181211 2

Fluorescent protein reporter genes are widely used to identify and sort murine pancreatic β-cells. In this study, we compared use of the MIP-GFP transgene, which exhibits aberrant expression of human growth hormone (hGH), with a newly derived Ins2<sup>Apple</sup> allele that lacks hGH expression on the expression of sex-specific genes. β-Cells from MIP-GFP transgenic mice exhibit changes in the expression of 7,733 genes, or greater than half of their transcriptome, compared with β-cells from Ins ...[more]

PMID: 30532991

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