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Effect of S. epidermidis phenol-soluble modulins on murine dorsal skin (24h)

ABSTRACT: To investigate the effect of both S. epidermidis phenol-soluble modulins (PSMs) and EcpA genetic knockouts on murine back skin. We then performed gene expression profiling analysis using data obtained from RNA-seq of 5 conditions (n=3 per condition).

ORGANISM(S): Mus musculus

PROVIDER: GSE210046 | GEO | 2023/07/01

REPOSITORIES: GEO

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Effect of S. epidermidis phenol-soluble modulins on primary human keratinocytes.

Project description:To investigate the effect of synthetic phenol-soluble modulins (PSMs) secreted by S. epidermidis on primary keratinocytes. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells at two time points.

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To identify the phenol soluble modulins whose production are primarily affected by SarZ in Staphylococcus epidermidis

Project description:Phenol-soluble modulins (PSMs) are a class of short, amphipathic, α-helical peptides, which have strong cytolytic effects toward leukocytes and erythrocytes in S. epidermidis. SarZ is a key transcriptional regulator required for the expression of virulence factors. To determine whether the production of PSMs is under the control of SarZ, a sarZ deletion mutant strain was generated. Through butanol extraction of the spent medium and subsequent HPLC-MS/MS analysis, the amounts of PSMs were compared between the sarZ mutant and its parent strain.

2024-12-05 | PXD057952 | Pride

Effect of S. epidermidis phenol-soluble modulins on murine dorsal skin (24h)

Project description:Effect of S. epidermidis phenol-soluble modulins on murine dorsal skin (24h)

| PRJNA863579 | ENA

Effect of S. epidermidis phenol-soluble modulins on primary human keratinocytes.

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AI-2-dependent gene regulation in Staphylococcus epidermidis

Project description:Autoinducer 2 (AI-2), a widespread by-product of the LuxS-catalyzed S-ribosylhomocysteine cleavage reaction in the activated methyl cycle, has been suggested to serve as an intra- and interspecies signaling molecule, but in many bacteria AI-2 control of gene expression is not completely understood. Particularly, we have a lack of knowledge about AI-2 signaling in the important human pathogens Staphylococcus aureus and S. epidermidis. Here, to determine the role of LuxS and AI-2 in S. epidermidis, we analyzed genome-wide changes in gene expression in an S. epidermidis luxS mutant and after addition of AI-2 synthesized by over-expressed S. epidermidis Pfs and LuxS enzymes. Genes under AI-2 control included mostly genes involved in sugar, nucleotide, amino acid, and nitrogen metabolism, but also virulence-associated genes coding for lipase and bacterial apoptosis proteins. In addition, we demonstrate by liquid chromatography/mass-spectrometry of culture filtrates that the pro-inflammatory phenol-soluble modulin (PSM) peptides, key virulence factors of S. epidermidis, are under luxS/AI-2 control. Our results provide a detailed molecular basis for the role of LuxS in S. epidermidis virulence and suggest a signaling function for AI-2 in this bacterium. Keywords: wild type without glucose control vs luxS mutant vs luxS mutant with auto-inducer II

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AI-2-dependent gene regulation in Staphylococcus epidermidis

2008-10-10 | E-GEOD-9427 | biostudies-arrayexpress

phenol degrading consortium

Project description:Phenol bioremediation from groundwater

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Characterization of Staphylococcus epidermidis biofilm-released cells (BRC) transcriptome upon interaction with whole human blood and its cellular and soluble components

Project description:The release of cells from S. epidermidis biofilms formed on medical devices has been associated with the onset of bloodstream infections resulting in increased morbidity and mortality among infected patients. In order to better understand the role of BRC in the pathogenesis of S. epidermidis biofilm-related infections, the transcriptome of these cells was evaluated upon exposure to human blood components. The major alterations observed were involved in the biosynthesis and metabolism of amino acids and import and export of substances through ABC transporters. In addition, the transcription of genes involved in biotin metabolism was found significantly up-regulated suggesting this pathway as a possible target for future therapeutic strategies.

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