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Effect of S. epidermidis phenol-soluble modulins on primary human keratinocytes.

ABSTRACT: Effect of S. epidermidis phenol-soluble modulins on primary human keratinocytes.

PROVIDER: PRJNA861992 | ENA |

REPOSITORIES: ENA

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Effect of S. epidermidis phenol-soluble modulins on primary human keratinocytes.

Project description:To investigate the effect of synthetic phenol-soluble modulins (PSMs) secreted by S. epidermidis on primary keratinocytes. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells at two time points.

2023-01-01 | GSE209653 | GEO

Effect of S. epidermidis phenol-soluble modulins on murine dorsal skin (24h)

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Effect of S. epidermidis phenol-soluble modulins on murine dorsal skin (24h)

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RNA-Seq analysis of human keratinocytes stimulated with synthetic PSMα3

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AI-2-dependent gene regulation in Staphylococcus epidermidis

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2008-10-10 | E-GEOD-9427 | biostudies-arrayexpress

AI-2-dependent gene regulation in Staphylococcus epidermidis

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VZV infection of primary keratinocytes

Project description:VZV infection of primary keratinocytes

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Next Generation Sequencing Facilitates Quantitative Analysis of Primary Keratinocytes and OSM-treated Primary Keratinocytes Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare transcriptome profiling (RNA-seq) between Primary Keratinocytes and OSM-treated Primary Keratinocytes Methods: mRNA profiles of Primary Keratinocytes and OSM-treated Primary Keratinocytes were generated by deep sequencing, in triplicate, using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks.

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microRNA profiling of primary human keratinocytes and primary human dermal fibroblasts

Project description:In search for factors, overexpression of which in human dermal fibroblasts causes direct conversion to cells similar to keratinocytes, micro RNA expression profiles of human primary keratinocytes and human primary dermal fibroblasts are investigated. Skin samples obtained from 3 different sites of 1 subject were used for establishment of 3 primary keratinocytes and 3 primary dermal fibroblasts. Thus obtained 3 primary keratinocytes and primary dermal fibroblasts underwent micro RNA profiling.

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