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Effect of S. epidermidis phenol-soluble modulins on primary human keratinocytes.

ABSTRACT: Effect of S. epidermidis phenol-soluble modulins on primary human keratinocytes.

PROVIDER: PRJNA861992 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20614753_1.fastq.gz	Fastqsanger.gz
	SRR20614753_2.fastq.gz	Fastqsanger.gz
	SRR20614754_1.fastq.gz	Fastqsanger.gz
	SRR20614754_2.fastq.gz	Fastqsanger.gz
	SRR20614755_1.fastq.gz	Fastqsanger.gz

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Effect of S. epidermidis phenol-soluble modulins on primary human keratinocytes.

Project description:To investigate the effect of synthetic phenol-soluble modulins (PSMs) secreted by S. epidermidis on primary keratinocytes. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells at two time points.

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Effect of S. epidermidis phenol-soluble modulins on murine dorsal skin (24h)

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To identify the phenol soluble modulins whose production are primarily affected by SarZ in Staphylococcus epidermidis

Project description:Phenol-soluble modulins (PSMs) are a class of short, amphipathic, α-helical peptides, which have strong cytolytic effects toward leukocytes and erythrocytes in S. epidermidis. SarZ is a key transcriptional regulator required for the expression of virulence factors. To determine whether the production of PSMs is under the control of SarZ, a sarZ deletion mutant strain was generated. Through butanol extraction of the spent medium and subsequent HPLC-MS/MS analysis, the amounts of PSMs were compared between the sarZ mutant and its parent strain.

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Effect of S. epidermidis phenol-soluble modulins on murine dorsal skin (24h)

Project description:Effect of S. epidermidis phenol-soluble modulins on murine dorsal skin (24h)

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AI-2-dependent gene regulation in Staphylococcus epidermidis

Project description:Autoinducer 2 (AI-2), a widespread by-product of the LuxS-catalyzed S-ribosylhomocysteine cleavage reaction in the activated methyl cycle, has been suggested to serve as an intra- and interspecies signaling molecule, but in many bacteria AI-2 control of gene expression is not completely understood. Particularly, we have a lack of knowledge about AI-2 signaling in the important human pathogens Staphylococcus aureus and S. epidermidis. Here, to determine the role of LuxS and AI-2 in S. epidermidis, we analyzed genome-wide changes in gene expression in an S. epidermidis luxS mutant and after addition of AI-2 synthesized by over-expressed S. epidermidis Pfs and LuxS enzymes. Genes under AI-2 control included mostly genes involved in sugar, nucleotide, amino acid, and nitrogen metabolism, but also virulence-associated genes coding for lipase and bacterial apoptosis proteins. In addition, we demonstrate by liquid chromatography/mass-spectrometry of culture filtrates that the pro-inflammatory phenol-soluble modulin (PSM) peptides, key virulence factors of S. epidermidis, are under luxS/AI-2 control. Our results provide a detailed molecular basis for the role of LuxS in S. epidermidis virulence and suggest a signaling function for AI-2 in this bacterium. Keywords: wild type without glucose control vs luxS mutant vs luxS mutant with auto-inducer II wild type without glucose control vs luxS mutant vs luxS mutant with auto-inducer II

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VZV infection of primary keratinocytes

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Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare transcriptome profiling (RNA-seq) between Primary Keratinocytes and OSM-treated Primary Keratinocytes Methods: mRNA profiles of Primary Keratinocytes and OSM-treated Primary Keratinocytes were generated by deep sequencing, in triplicate, using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks.

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