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Comprehensive analyses of small RNAs associated with C. elegans argonautes

ABSTRACT: To genome-widely investigate functions of C. elegans argonautes (AGOs), we applied CRISPR/Cas9 technology to introduce an in-frame GFP::FLAG multiplex tag into the endogenous locus of C. elegans AGOs and performed RNA immunoprecipitation (IP) with anti-GFP or anti-FLAG antibody, followed by unique molecular identifier (UMI)-mediated cDNA library constructions and subjected to high-throughput sequencing. We obtained targets of C. elegans AGOs analyzed from small RNA reads of input and 19 AGO IP samples.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE212382 | GEO | 2023/02/13

REPOSITORIES: GEO

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Comprehensive analyses of small RNAs associated with C. elegans argonautes

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