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Expression profiling of genes responding to flurbiprofen enantiomers in HaCaT cells

ABSTRACT: Previous work from our laboratory has identified R-flurbiprofen as a candidate chemopreventive agent for skin cancer. Despite our findings, and the investigation of R-flurbiprofen as a chemopreventive for prostate and colon cancer by other groups, the mechanism of action remains unclear. To gain mechanistic insight into these anti-proliferative effects, we carried out microarray analysis of HaCaT cells in response to both R-flurbiprofen and S-flurbiprofen treatment. This analysis shows that both R-flurbiprofen and S-flurbiprofen produced significant changes in gene expression compared to untreated control, however, no significant differences were identified between effects induced by the individual enantiomers. Many of the genes found to be altered compared to control are implicated in cellular growth, apoptosis and metastasis, a selection of which are discussed. The altered expression of these genes may contribute to the anti-proliferative effects of R-flurbiprofen and S-flurbiprofen. Keywords: oligonucleotide array, HaCaT, flurbiprofen, NSAID

ORGANISM(S): Homo sapiens

PROVIDER: GSE21274 | GEO | 2010/05/09

SECONDARY ACCESSION(S): PRJNA126265

REPOSITORIES: GEO

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