Transcriptomics

Dataset Information

0

Purification-sequencing of RNA super-assemblies.


ABSTRACT: C. elegans oogenesis arrest offers a unique and biologically relevant opportunity to investigate how condensation reorganizes the transcriptome in the context of cellular adaptation to quiescence. To investigate how RNA self-organization into condensates controls mRNA cytosolic stoichiometries depending on sequence identity and translational activity, we adapted a new Fluorescent Activated Particle Sorting (FAPS) method to selectively sort germline P-bodies from C. elegans oocytes. Fluorescently labelled in oocytes, GFP:CAR-1 P-bodies were formaldehyde fixed and FAPS sorted from whole animal extracts by their size and fluorescence. We then performed gene expression profiling analysis using data obtained from RNA-seq of oocyte P-bodies, dissected oocytes and whole animal extracts.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE213222 | GEO | 2023/09/12

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2017-09-01 | E-MTAB-5477 | biostudies-arrayexpress
2023-04-24 | PXD040119 | JPOST Repository
2011-12-05 | E-GEOD-31602 | biostudies-arrayexpress
2024-07-30 | E-MTAB-12923 | biostudies-arrayexpress
2014-07-22 | E-GEOD-49828 | biostudies-arrayexpress
2024-07-01 | GSE261784 | GEO
| PRJNA342483 | ENA
2022-01-02 | GSE189296 | GEO
2011-10-12 | E-GEOD-32689 | biostudies-arrayexpress
2010-09-01 | GSE23447 | GEO