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Quantitative proteomic analysis of p62-body components isolated by FAPS method.

ABSTRACT: We developed a method using fluorescence-activated particle sorting (FAPS) to purify p62-bodies efficiently and identified several p62-body components, including known not only autophagy substrates and receptors.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yoshiki Miura

PROVIDER: PXD040119 | JPOST Repository | Mon Apr 24 00:00:00 BST 2023

REPOSITORIES: jPOST

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			Action	DRS
	20230123%20Quantification.xlsx	Xlsx
	20230123%20identification.xlsx	Xlsx
	22-691.raw	Raw
	22-691_realtimesearch.tsv	Tabular
	22-692.raw	Raw

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Proteomic analysis of p62-body components isolated by FAPS method.

Project description:Here, we developed a method using fluorescence-activated particle sorting (FAPS) to purify p62-bodies efficiently and identified several p62-body components, including known not only autophagy substrates and receptors, but also subunits of supramolecular protein complexes such as vault, a large cytoplasmic ribonucleoprotein particle, and clathrin-coated vesicles.

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