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Dynamic network-guided CRISPRi screen reveals CTCF loop constrained enhancer function in cell state transitions

ABSTRACT: We performed CRISPRi enhancer screens during differentiation of human embryonic stem cells (hESC) to definitive endoderm (DE) in genomic windows surrounding ten lineage-specifying core TFs. We also generated scRNA-seq, ATAC-seq, and TF ChIP-seq data and show that CTCF loops constrain the interactions between enhancers and their promoter targets.

ORGANISM(S): Homo sapiens

PROVIDER: GSE213394 | GEO | 2022/10/01

REPOSITORIES: GEO

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