Transcriptomics

Dataset Information

0

RNA Interacts with Topoisomerase I to Modulate its Catalytic Activity


ABSTRACT: Topoisomerase I (TOP1) is an essential enzyme that relaxes DNA to prevent and dissipate torsional stress during transcription. However, the mechanisms underlying the regulation of TOP1 activity remain elusive. Using eCLIP and UV-RIP-seq we identify TOP1 as a RNA binding protein (RBP). Using a TOP1 RNA binding mutant and TOP1cc-seq to map TOP1 catalytic activity, we reveal that RNA opposes TOP1 activity as RNAPII commences transcription of active genes. These findings support new insights into the coordinated actions of RNA and TOP1 in regulating DNA topological stress intrinsic to RNAPII-dependent transcription.

ORGANISM(S): Homo sapiens

PROVIDER: GSE213403 | GEO | 2024/08/21

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2011-07-21 | E-GEOD-23677 | biostudies-arrayexpress
2011-07-22 | GSE23677 | GEO
2013-04-17 | E-GEOD-37357 | biostudies-arrayexpress
2013-04-17 | GSE37352 | GEO
2013-04-17 | GSE37357 | GEO
2013-04-17 | E-GEOD-37352 | biostudies-arrayexpress
2016-04-12 | GSE57628 | GEO
2019-06-22 | GSE128798 | GEO
2021-11-11 | GSE171132 | GEO
2020-03-13 | PXD017239 | Pride