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Repertoire analysis of the repertoire of insulin-reactive B cells in type 1 diabetes-prone mice

ABSTRACT: Seropositivity for autoantibodies against islet autoantigens is associated with the development of autoimmune type 1 diabetes and B cell targeted therapies are effective in both mouse models and in patients who are affected by or at risk for autoimmune type 1 diabetes. The role of B cell receptor affinity in autoimmune type 1 diabetes is unclear. Here, we employed single cell RNA sequencing to define the relationship between B cell receptor affinity for insulin and B cell phenotype during disease development using immunoglobulin heavy chain (VH125) transgenic mouse model (VH125.NOD) in which insulin binding B cells lose self-tolerance, becoming activated during development of autoimmmun type 1 diabetes.

ORGANISM(S): Mus musculus

PROVIDER: GSE213973 | GEO | 2022/09/26

REPOSITORIES: GEO

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