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MiRNA-150_R-1 mediates the HIF-1/ErbB signaling pathway to regulate the adhesion of endometrial epithelial cells in cows experiencing retained placenta

ABSTRACT: In this study, we performed RNA-sequencing to characterize gene expression profiles in caudal vein blood samples of postpartum Holstein cows whose fetal membranes were discharged normally or retained to identify retained placenta (RP)-related genes and evaluate their molecular mechanisms.

ORGANISM(S): Bos taurus

PROVIDER: GSE214588 | GEO | 2022/10/05

REPOSITORIES: GEO

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circAMN1/miR-205_R-1/SLC39A8 axis regulates ferroptosis in endometrial epithelial cells and regulates the occurrence of retained placenta in cows

Project description:In this study, we performed RNA-sequencing to characterize the expression profiles of circRNAs in caudal vein blood samples of postpartum Holstein cows whose fetal membranes were discharged normally or retained to identify retained placenta (RP)-related genes and evaluate their molecular mechanisms.

2023-11-24 | GSE248362 | GEO

Proteome dataset of liver from dairy cows experiencing negative or positive energy balance at early lactation

Project description:This article contains raw and processed data related to research published by Swartz et al. [1]. Proteomics data from liver of postpartum dairy cows were obtained by liquid chromatography-mass spectrometry following protein extraction. Differential abundance between liver of cows experiencing either negative energy balance (NEB, n=6) or positive energy balance (PEB, n=4) at 17±3 DIM was quantified using MS1 intensity based label-free. There is a paucity of studies examining the associations of NEB with the liver proteome in early lactation dairy cows. Therefore, our objective was to characterize the differences in the liver proteome in periparturient dairy cows experiencing naturally occurring NEB compared to cows in PEB. In this study, multiparous Holstein dairy cows were milked either 2 or 3 times daily for the first 30 days in milk (DIM) to alter energy balance, and were classified retrospectively as NEB (n=18) or PEB (n=22). Liver biopsies were collected from 10 cows (n=5 from each milking frequency), that were retrospectively classified according to their energy balance (NEB, n=6; PEB, n=4). The liver proteome was characterized using label-free quantitative shotgun proteomics. This novel dataset contains 2,741 proteins were identified, and 68 of those were differentially abundant between NEB and PEB (P≤0.05 and FC±1.5); these findings are discussed in our recent research article [1]. The present dataset of liver proteome can be used as either biological markers for disease or therapeutic targets to improve metabolic adaptations to lactation in postpartum dairy cattle.

2021-10-13 | PXD028124 | Pride

Biomarkers for diagnosing the retained placenta in cows-does the analysis of serum proteome keep the secret?

Project description:Retained placenta (RP), a quite common disorder in dairy cows, shows a high negative impact on their health status and milk production. Therefore, the biomarkers indicating the risk for RP could yield benefits in dairy management. The study aimed to investigate the serum proteome as the source of the biomarkers for diagnosing the RP. Using the high-resolution liquid chromatography-tandem mass spectrometry, we compared the composition of serum proteome between 9 cows with the RP and 6 with the physiologic puerperium (PP). Among 651 identified proteins, seven had higher, and three showed lower abundance in RP than in the PP group. The differently abundant proteins participated in 15 pathways: six related to hemostasis, three involved in the lipoprotein metabolism, and the remaining ones associated with redox homeostasis, post-translational modification, scavenging, etc. Validation of the proteomic results, using ELISA and spectrophotometric assays, showed that the two identified proteins, haptoglobin, and lipopolysaccharide-binding protein, could represent reliable RP biomarkers. Our results confirmed that the analysis of serum proteome shed "the light" on the counterparty piece(s) of the RP pathogenesis "puzzle" and could be an efficient "tool" in mining for RP biomarkers.

2023-01-13 | PXD034963 | Pride

Stranded Total RNA-seq of liver biopsy collected from Holstein Friesian cows

Project description:To explore potential functional genes related to milk production traits in cow, we performed total RNA-seq using liver biospy collected from 77 Holstein Friesian cows at postpartum 2 weeks.

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Gene expression in extra-embryonic tissues flushed out at D18 from heifers, early and late lactating cows

2014-02-04 | GSE46940 | GEO

Gene expression in extra-embryonic tissues flushed out at D18 from heifers, early and late lactating cows

Project description:Evaluate the influence of maternal metabolism on gene expression profiles from extra-embryonic tissues at D18 Keyword: Holstein Heifers and Postpartum dairy cows, metabolism and energy status, elongation and gastrulation, extra-embryonic tissues, transcriptome, correlations between genes & metabolites? 8 replicates in GH, 11 replicates in ELC and 10 replicates in LLC (extra-embryonic tissues) per group of female (N=3) were hybridized (total N=29) against a placental reference that hybridized itself to 99% of the array. Hybridization design: 6 microarrays per experiment (N=5) were randomly hybridized to the EET from 2 conceptuses originating either from development in heifers, ELC or LLC. Extra-embryonic tissues were systematically labelled with Cy3 and Reference in Cy5.

2014-02-04 | E-GEOD-46940 | biostudies-arrayexpress

RNA-seq study on the placentomes collected from the cows with or without retained placenta

Project description:We conducted an RNA-sequencing on the placentomes that were collected from 4 and 3 cows with and without retained placenta, respectively. Placentomes were collected from the cows within 30 minutes after parturition and samples were taken from the feto-maternal junction sites of the placentomes. These samples were used for RNA-sequencing and the results showed 240 genes that were differentially expressed between the two groups. These genes could be candidate genes that are involved in the development of placental retention in cattle.

2022-08-01 | GSE194033 | GEO

Influence of in-vitro culture conditions and maternal metabolic status during embryonic genome activation on the Day-4 embryo transcriptome

Project description:Milk production during the early postpartum in dairy cows can impact the circulating metabolites and negatively affects embryo survival. However, the molecular consequences for the embryo during its development in the oviduct are still unknown. The objective was to determine the impact of metabolic status on embryonic genome activation (EGA) using high-throughput sequencing to generate comprehensive transcriptome profiles of bovine 16-cell stage embryos which had undergone EGA in vitro or in the oviducts of primiparous postpartum dry or postpartum lactating Holstein Friesian cows.

2023-08-30 | GSE226844 | GEO

Negative energy balance and hepatic gene expression patterns in high yielding dairy cows during the early postpartum period [liver]

Project description:In high yielding dairy cows the liver undergoes extensive physiological and biochemical changes during the early postpartum period in an effort to re-establish metabolic homeostasis and to counteract the adverse effects of negative energy balance (NEB). These adaptations are likely to be mediated by significant alterations in hepatic gene expression. To gain new insights into these events an EB model was created using differential feeding and milking regimes to produce two groups of cows with either a mild (MNEB) (n=5) or severe NEB (SNEB) (n=6) status. Cows were slaughtered and liver tissues collected on days 6-7 of the first follicular wave postpartum. Using an Affymetrix® 23k oligonucleotide bovine array to determine global gene expression in hepatic tissue of these cows, a total of 416 genes (189 up- and 227 down-regulated) were found to be altered by SNEB. Network analysis using Ingenuity Pathway Analysis revealed that SNEB was associated with widespread changes in gene expression classified into 36 gene networks including those associated with lipid metabolism, connective tissue development and function, cell signalling, cell cycle and metabolic diseases. Severe NEB cows displayed reduced expression of transcription activators and signal transducers that regulate the expression of genes and gene networks associated with cell signalling and tissue repair. These alterations are linked with increased expression of abnormal cell cycle and cellular proliferation associated pathways. This study provides new information and insights on the effect of SNEB on gene expression in high yielding Holstein Friesian dairy cows in the early postpartum period.

2014-04-08 | GSE56547 | GEO

Effect of heat stress on serum enzyme activity, antioxidant capacity and immune parameter metabolomics datasets in Holstein cows at different growth stages

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2022-08-11 | MTBLS5148 | MetaboLights

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