Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The Groucho/Tle coregulators are essential for early thymic progenitors and T-cell lineage specification [RNA-seq]

ABSTRACT: The goal of this study is to determine the functional requirements for Groucho/Tle coregulators in early stages of T cell development. By ablating three mammalian homologues, Tle1, Tle3, and Tle4 genes, we found that Tle1/3/4 are essential for repressing non-T cell lineage potential in early thymic progenitors, and therefore pivotal for T-cell lineage specification.

ORGANISM(S): Mus musculus

PROVIDER: GSE217025 | GEO | 2022/11/06

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

The Groucho/Tle coregulators are essential for early thymic progenitors and T-cell lineage specification [Cut&Run]

Project description:The goal of this study is to determine the functional requirements for Groucho/Tle coregulators in early stages of T cell development. By ablating three mammalian homologues, Tle1, Tle3, and Tle4 genes, we found that Tle1/3/4 are essential for repressing non-T cell lineage potential in early thymic progenitors, and therefore pivotal for T-cell lineage specification.

2022-11-06 | GSE217024 | GEO

The Groucho/Tle coregulators are essential for early thymic progenitors and T-cell lineage specification [ATAC-seq]

2022-11-06 | GSE217023 | GEO

The Groucho/Tle coregulators are essential for early thymic progenitors and T-cell lineage specification

Project description:The Groucho/Tle coregulators are essential for early thymic progenitors and T-cell lineage specification

| PRJNA896584 | ENA

The Groucho/Tle coregulators are essential for early thymic progenitors and T-cell lineage specification [ATAC-seq]

Project description:The Groucho/Tle coregulators are essential for early thymic progenitors and T-cell lineage specification [ATAC-seq]

| PRJNA896588 | ENA

The Groucho/Tle coregulators are essential for early thymic progenitors and T-cell lineage specification [Cut&Run]

Project description:The Groucho/Tle coregulators are essential for early thymic progenitors and T-cell lineage specification [Cut&Run]

| PRJNA896589 | ENA

The Groucho/Tle coregulators are essential for early thymic progenitors and T-cell lineage specification [RNA-seq]

Project description:The Groucho/Tle coregulators are essential for early thymic progenitors and T-cell lineage specification [RNA-seq]

| PRJNA896590 | ENA

Impact of Tle1, Tle3, and Tle4 deficiency on hematopoietic stem cells (HSCs) [RNA-seq]

Project description:To compare the transcriptomes between control and Tle1/3/4-deficient HSCs Tle co-repressors can co-opt a number of transcription factors to repress target gene expression. Due to gene duplication during evolution, there are 4 Tle genes in mammalian genome, and their functional redundancy has a major hurdle to understand their roles in hematopoietic stem cells (HSCs). Here we used Vav-Cre to ablate Tle1, 3, and 4 genes in HSCs, and performed RNA-seq to determine the impact of Tle deficiency on HSCs. To further define the regulatory mechanisms, we performed Tle3 ChIPseq on c-Kit+ hematopoietic progenitor cells. The data obtained collectively indicate a critical, intrinsic requirement for Tle corepressors in HSC self-renewal.

2020-02-01 | GSE126271 | GEO

Genome-wide maps of Tle3 binding in hematopoietic progenitor cells [ChIP-seq]

Project description:To determine Genome-wide Tle3 binding locations in c-Kit+ mouse bone marrow cells Tle co-repressors can co-opt a number of transcription factors to repress target gene expression. Due to gene duplication during evolution, there are 4 Tle genes in mammalian genome, and their functional redundancy has a major hurdle to understand their roles in hematopoietic stem cells (HSCs). Here we used Vav-Cre to ablate Tle1, 3, and 4 genes in HSCs, and performed RNA-seq to determine the impact of Tle deficiency on HSCs. To further define the regulatory mechanisms, we performed Tle3 ChIPseq on c-Kit+ hematopoietic progenitor cells. The data obtained collectively indicate a critical, intrinsic requirement for Tle corepressors in HSC self-renewal.

2020-02-01 | GSE126270 | GEO

Enhancer binding of RUNX1 and Groucho family repressor TLE3 is stabilized by FOXC1 to block differentiation in acute myeloid leukemia [ChIP-Seq]

Project description:A differentiation block is the cardinal pathologic feature of acute myeloid leukaemia (AML) but the underlying mechanisms are incompletely understood. Despite absent expression in normal hematopoietic lineages, the Forkhead family transcription factor FOXC1, which is a critical regulator of normal mesenchymal and mesodermal differentiation, is highly expressed in ~20% of cases of AML where it confers a block to monocyte/macrophage lineage differentiation. Through integrated proteomics and bioinformatics approaches, we discovered that FOXC1 interacts with RUNX1 through its Forkhead DNA binding domain and that the two factors co-occupy a discrete set of primed or active enhancers distributed close to monocyte/macrophage differentiation genes. FOXC1 stabilises association of RUNX1, HDAC1 and the Groucho family repressor protein TLE3 at these sites. FOXC1 knockdown induced loss of these repressor proteins and gain of CEBPA binding, with localised increase in enhancer acetylation; nearby genes, including KLF2, were up regulated. A genome-wide redistribution of RUNX1 and TLE3 binding from enhancers to promoters was triggered by FOXC1 knockdown leading to repression of self-renewal genes including MYC and MYB. Thus, FOXC1 misexpression confers a differentiation block in AML by stabilising recruitment of a RUNX1/HDAC1/TLE3 repressor complex at primed or active enhancers close to genes which control monocyte/macrophage lineage differentiation.

2021-08-13 | GSE159691 | GEO

Enhancer binding of RUNX1 and Groucho family repressor TLE3 is stabilized by FOXC1 to block differentiation in acute myeloid leukemia [RNA-Seq]

2021-08-13 | GSE159690 | GEO